蛇雕线粒体DNA全序列测定与分析  

Complete Sequence Determination and Analysis of Spilornis cheela mtDNA

作　　者：覃屏生[1,2] 石菁萍[1,2] 管清新[1,2] 曾德龙[1,2] 秦新民[1,2] 

机构地区：[1]广西师范大学生命科学学院,广西桂林541004 [2]广西珍稀濒危动物生态学重点实验室,广西桂林541004

出　　处：《广西师范大学学报（自然科学版）》2013年第4期115-120,共6页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金资助项目(31160071);广西珍稀濒危动物生态学重点实验室资助项目(桂科能1301Z004)

摘　　要：蛇雕为我国二级珍稀濒危物种。本文采用PCR扩增方法测定蛇雕Spilornischeela线粒体DNA全序列,结果:该DNA全长18291bp,为双链环状DNA分子,含有13个蛋白质编码基因、2个rRNA基因、22个tRNA基因、1个控制区(D-loop)和1个假设控制区(pseudo-controlregion),其基因排序与已报道的其他猛禽鸟类排列方式相同。该基因组基因DNA排列紧密,共观察到53bp基因间隔(除控制区1144bp和假控制区1532bp外)与31bp基因重叠;全序列AT含量为53.02%;13个蛋白编码基因中,除COI使用GTG作为起始密码子外,其余使用ATN。此外,10个蛋白编码基因使用常规三联终止密码子TAA、AGG和TAG作为终止密码子,其余3个基因为不完全终止密码子T。除tRNA-Ser(AGY)外,其余21个tRNA基因的二级结构均为典型的三叶草结构。D-loop存在2个结构区:中央保守区(CSB-F、CSB-D和CSB-C)和保守序列区(CSB-1)。在假控制区的3′端有一个48bp的8个重复串联单元。Spilornis cheela （Ayes ,Falconiformes,Accipitridae） is considered as endemic raptor species and listed in the second category of National Key Protected Wild Animals in China. In an attempt to improve molecular knowledge about it,a complete mtDNA was sequenced in this study. The mtDNA is a circular molecule with 18 291 bp and consists of 13 protein-coding genes （PCGs）,22 tRNA genes,2 ribosomal RNA genes,1 control region （D-loop） and a pseudo-control region （ψCR）,which share the same arrangement with most of reported raptor species. The 37 genes are closely assembled one after the other. Only 59 bp （excluding control region 1 144 bp and pseudo-control region 1 532 bp） in intergenic spacers and 31 bp overlapped between several gene pairs. The nucleotide composition bias towards adenine and thymine with a content of A＋T of whole genome is 53.02%. Furthermore,13 PCGs used ATN as the start codon,except for CO I start with GTG. Besides,ten of the thirteen PCGs are terminated with the common triplet stop codon TAA,AGG and TAG,the remainders are stopped with incomplete stop codon （T）. The secondary structures of 22 tRNA were predicted,they can be fold in the typical cloverleaf structure described for vertebrate mitochdrial tRNAs,with the unique exception of tRNA-Ser （AGY）. Three central conservative blocks （CSB-F,CSB-D and CSB-C） and a conservative sequence block （CSB- 1） were located in the D-Loop. Additionally,a 48 bp tandem repeat was found at the 3r end of ψCR,consisting of eight repeats.

关 键 词：鹰科 蛇雕 线粒体基因组 

分 类 号：Q953[生物学—动物学] Q959.724