鸡包涵体肝炎免疫组织化学动态研究  

IMMUNOHISTOCHEMICAL DYNAMIC STUDIES ON INCLUSION BODY HEPATITIS IN CHICKENS

作　　者：刘建民[1] 胡维华[2] 徐福南[1] 刘聚祥[2] 武现军[2] 

机构地区：[1]南京农业大学动物医学院,210095 [2]河北农业大学动物科技学院,071001

出　　处：《畜牧兽医学报》2001年第1期38-43,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河北省自然科学基金资助项目

摘　　要：本试验对 10 0只 3日龄雏鸡用人工口服感染Ⅷ型腺病毒建立了鸡包涵体肝炎 (Inclusionbodyhepytitis IBH)模型后以免疫金银法进行免疫组织化学动态研究。结果表明 ,该病毒对鸡的肝脏、胸腺、法氏囊、脾脏、小肠、肾脏、胰腺等脏器的上皮细胞、淋巴细胞、血管内皮细胞 ,窦内皮细胞及红细胞等均具有亲嗜性 ,以肝脏、法氏囊、肾脏最为明显。此外 ,多数组织在接种 12h～ 2d及 9d～ 11d细胞核内两次出现胶体金颗粒 ,其形态表现为粗大颗粒及微细颗粒两种。Immunohistochemical dynamic changes of inclusion body hepatitis (IBH) were studied following the oral administration of a serotype 8 strain of avian adenovirus into 3 day old chickens.The results showed that the adenovirus had an affinity to the epithelia,lymphocytes,the blood vessel and sinus endothelium and erythrocytes in the livers,thymuses,bursas,spleens,intestines,kidneys and pancreases.In addition,during 12 h 2days,9 days 11 days post inoculation(pi),the nuclei of most organs were positive twice.The positive particles in the nuclei have two kinds of appearances:large and fine particular.

关 键 词：包涵体肝炎 免疫组织化学 发病机理 鸡 免疫金银法 

分 类 号：S858.315.3[农业科学—临床兽医学]