感染鸡贫血病毒雏鸡接种新城疫疫苗后免疫功能和免疫调节的变化  

STUDY ON IMMUNE FUNCTION AND IMMUNOREGULATION POST NEWCASTLE DISEASE VACCINATION OF CHICKENS INFECTED WITH CHICKEN ANEMIA VIRUS

作　　者：刘忠贵[1] 郑世民[1] 徐彦波[1] 周志勇[1] 杨丽萍[1] 李广兴[1] 阿哈买堤.买买提 

机构地区：[1]东北农业大学,哈尔滨150030

出　　处：《畜牧兽医学报》2001年第1期92-96,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目! (396 70 5 6 4)

摘　　要：雏鸡 1日龄感染鸡贫血病毒 ,8日龄接种Lasota疫苗 ,以未感染免疫雏鸡为对照 ,于免疫后7、14、2 8d检测血清免疫球蛋白IgG、IgM、IgA数量 ,血凝抑制抗体 (HI)滴度 ;胸腺、法氏囊、脾脏T细胞、IgG+、IgM+、IgA+抗体生成细胞数量及T、B细胞增殖反应 ;胸腺、脾脏细胞因子IL 2、IFN活性的变化。结果发现 ,感染CAV雏鸡Lasota疫苗免疫后 ,其血清IgG、IgM、IgA免疫球蛋白含量明显减少 ,HI抗体滴度降低 ;胸腺、法氏囊、脾脏T细胞、抗体生成细胞数量降低及T、B细胞增殖反应减弱 ,胸腺、脾脏IL 2及IFN诱生活性降低 ,表明其细胞免疫和体液免疫功能以及细胞因子免疫调节作用均较未感染免疫雏鸡明显减弱。Chickens were infected with chicken anemia virus(CAV) at one day old and vaccinated with Lasota vaccine 8 days later.Meanwhile,uninfected chickens were vaccinated as controls.At 7、14、28 days post vaccination,the content of IgG、IgM、IgA and HI titer in serum,the number of T cells,IgG +、IgM +、IgA + antibody producing cells in the thymus、bursa of Fabricius and spleen,the proliferative response of T、B cells,the inductive activity of interleukin 2(IL 2) and interferon (IFN) in the thymus and spleen were tested.It was discovered that the content of IgG、IgM、IgA and HI titer in serum,the number of T cells,IgG +、IgM +、IgA + antibody producing cells in the thymus,bursa of Fabricius and spleen,the proliferative respanse of T cells and B cells as well as the inductive activity of IL 2 and IFN in the thymus and spleen of infected vaccinated chickens significantly decreased than those of controls.These indicated that the immune function and immunoregulation were droped post ND vaccination of CAV infected chickens. Key words:CAV infected chicken;Lasota vaccine;T cell;Antibody producing cell;T.B cell proliferitive response;IL 2;IFNhe number of T cells,IgG +、IgM +、IgA + antibody producing cells in the thymus、bursa of Fabricius and spleen,the proliferative response of T、B cells,the inductive activity of interleukin 2(IL 2) and interferon (IFN) in the thymus and spleen were tested.It was discovered that the content of IgG、IgM、IgA and HI titer in serum,the number of T cells,IgG +、IgM +、IgA + antibody producing cells in the thymus,bursa of Fabricius and spleen,the proliferative respanse of T cells and B cells as well as the inductive activity of IL 2 and IFN in the thymus and spleen of infected vaccinated chickens significantly decreased

关 键 词：鸡 贫血病毒感染 新城疫 疫苗接种 免疫功能 免疫调节 

分 类 号：S858.315.3[农业科学—临床兽医学] S858.312.5[农业科学—兽医学]