改良大鼠子宫内膜异位症动物模型成模率的实验研究  

Experimental study of improved rat endometriosis molding rate

作　　者：马春燕[1] 王振国[2] 赵爱华[2] 万广志[1] 

机构地区：[1]河北北方学院,河北张家口075000 [2]中国人民解放军第309医院妇产科,北京100091

出　　处：《中国现代医学杂志》2013年第35期28-32,共5页China Journal of Modern Medicine

摘　　要：目的通过对首次造模未成功大鼠进行再次移植手术,探索提高大鼠子宫内膜异位症动物成模率的新手段。方法非动情期Wistar大鼠60只随机分为实验组和对照组,每组30只,通过自体移植方法复制子宫内膜异位症动物模型。4周后观察移植组织形态、颜色,测量移植组织体积并进行病理组织学分析。实验组首次造模未成功大鼠进行再次移植手术,操作过程与首次完全一致,对照组仅行二次开腹不进行移植。结果实验组与对照组总成模率分别为68.97%和43.33%,差异有统计学意义(P<0.05)。结论经二次移植手术,子宫内膜异位症动物造模成功率显著提高。【Objective】 To improve the success rate of rat EMs molding. 【Methods】 60 non-estrus wis- tar rats were randomly divided into experimental and control groups（n =30）. The two groups of rats were es- tablished the endometriosis model by autologous transplantation methods. Four weeks later, we observe graft morphology and color, measure the volume of the graft and analyze histopathologically. If the first time was not success, we conduct the second, the same to the first. 【Results】 The total successful rate of two groups were 68.97% and 43.33% respectively, there was significant difference（P 0.05）. 【Conclusion】 The second transplantation can significant increase the success rate of constructing rat model.

关 键 词：子宫内膜异位症 大鼠 动物模型 

分 类 号：R711.32[医药卫生—妇产科学]