诸氏鲻虾虎鱼染色体组型分析  

Study on the karyotype of Mugilogobius chulae

作　　者：陈小曲[1] 黄韧[1] 李建军[1] 

机构地区：[1]广东省实验动物监测所,广东省实验动物重点实验室,广东广州510663

出　　处：《热带海洋学报》2013年第6期88-95,共8页Journal of Tropical Oceanography

基　　金：广东省科技计划项目(2007B080501004;2009B060300011;2010A040209005;2010B090301027)

摘　　要：通过研究诸氏鲻虾虎鱼(Mugilogobius chulae)的染色体,分析了该鱼的细胞遗传学特征。取该鱼胚胎细胞,经秋水仙素浸泡、低渗处理、卡诺氏固定液固定后,用常规空气干燥法制作染色体标本,吉姆萨(Giemsa)染色后对获得的中期分裂相进行核型分析。研究结果表明,诸氏鲻虾虎鱼的染色体数目(2n)为44,核型为10m+10sm+4st+20t,染色体总臂数(NF)为64,未发现多倍体现象,也未发现异型性染色体和随体。In this study, the karyotype of Mugilogobius chulae was firstly reported. The examined materials are metaphase of the early embryo cells prepared by the colchicine-hypotonic-air drying technique. The results showed that M. chulae has 2n=44 diploid chromosomes and the karyotype of 2n= 10m＋10sm＋4st＋20t with its fundamental arm number （NF） =64. Neither the polyploidy chromosomes nor the sex chromosomes were found, and there was no visible evidence of the satellites.

关 键 词：诸氏鲻虾虎鱼 染色体 组型 

分 类 号：P735.541[天文地球—海洋生物学] Q343.2[天文地球—海洋科学]