缺血再灌注对心肌细胞Ca^(2+)-ATP酶蛋白及其基因表达的影响  

The effects of ischemic reperfusion on protein and gene expression of sarcoplasmic reticulum Ca^(2+)-ATPase in rat myocyte

作　　者：陈群清[1] 闫玉生[1] 童健[1] 朱平[1] 

机构地区：[1]第一军医大学珠江医院心胸外科,广州510282

出　　处：《广东医学》2001年第1期19-21,共3页Guangdong Medical Journal

基　　金：广东省自然科学基金资助项目(项目编号994084)

摘　　要：目的 研究鼠缺血再灌注（Ｉ／Ｒ）心肌细胞肌浆网钙ＡＴＰ酶（ＳＲ·Ｃａ２＋－ＡＴＰａｓｅ，ＳＥＲＣＡ２ａ）的蛋白及其基因表达的变化，探讨基固调控在。肌保护中的作用。方法 将ＳＤ大鼠分为正常对照组与Ｉ／Ｒ组，利用离体心工作模型进行灌注，采用Ｗｅｓｔｅｒｎ－Ｂｌｏｔ方法检测心肌细胞ＳＥＲＣＡ２ａ蛋白的含量，利用Ｎｏｒｔｈｅｒｎ－Ｂｌｏｔ方法测定ＳＥＲＣＡ２ａ ｍＲＮＡ相对含量。结果Ｉ／Ｒ心肌细胞的ＳＥＲＣＡ２ａ蛋白含量（０．５３８±０．００６ｖｓ１．０２０±０．０１５）及其ｍＲＮＡ相对量（０６２±０．００８ｖｓ０．９５±０．００９）均明显下降，分别下降３４．７％与４７．３％，其变化状态与其心肌功能变化一致。结抡 缺血再灌注过程严重影响了心肌细胞 ＳＲ·Ｃａ２＋－ＡＴＰａｓｅ蛋白及其基因的表达，ＳＥＲＣＡ２ａ基因表达显著下降可能是心肌Ｉ／Ｒ损伤的重要机制；若采取有效措施进行基固调控，增强ＳＥＲＣＡ２ａ基因的表达，可能起到保护Ｉ／Ｒ心肌细胞、增强心肌功能的作用。Objective To observe the changes of sarcoplasmic reticulum Ca2+ - ATPase (SERCA2a) protein and gene expression in ischemic reperfusion (I/R) myocytes; To reveal the effects of gene regulation in myocardial protection. Methods Rats were divided into two groups (normal control group and I/R group) , An isolated rat heart working model was used, to determine the relative levels of SERCA2a protein and mRNA in different groups by Western Blot and Northern Blot analysis. Results The protein and mRNA levels declined markedly in the I/R group (0.538 ± 0.006 vs 1.020 ± 0.015 in protein, 0.62 ± 0.008vs 0.95 ± 0.009 in mRNA, P<0.01) for 34.7% and 47.3% respectively, The changes were correlated closely with myocardial function. Conclusion Ischemic reperfusion may adversely affect SERCA2a expression on protein and gene level, Depressed SERCA2a expression in I/R course may be an important mechanism in myocardial ischemic reperfusion injury. It may be helpful to protect myocytes from ischemic reperfusion injury and to improve myocardial function by upregulating gene expression.

关 键 词：心肌缺血 再灌注损伤 心肌保护 CA^2+-ATP酶 基因表达 

分 类 号：R542.202[医药卫生—心血管疾病]