海藻酸钠涂敷手性固定相的制备及色谱评价  

Preparation and evaluation of a chiral stationary phase based on sodium alginate in HPLC

作　　者：李媛媛[1] 孙维维[1] 飞志欣 袁黎明[1] 

机构地区：[1]云南师范大学化学化工学院,昆明650500

出　　处：《分析试验室》2014年第3期254-256,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(21127012)资助

摘　　要：将海藻酸钠的乙酸溶液涂敷在3-氨丙基三乙氧基硅胶上,合成和表征了海藻酸钠涂敷手性固定相,以不同比例的正己烷/异丙醇为流动相,研究了正相色谱条件下海藻酸钠涂敷手性固定相对10种手性化合物对映体及3种位置异构体的拆分能力。在常用流动相条件下,有6种手性化合物对映体(3-苄氧基-1,2-丙二醇、DNB-亮氨酸、1-(1-萘基)-乙醇、联糠醛、阿普洛尔、反-1,2二苯环氧乙烷)和2种位置异构体(o,m,p-苯二酚、o,m,p-二溴苯)得到不同程度的拆分,表明海藻酸钠涂敷手性固定相手性识别率较高,手性识别能力较好。After sodium alginate was dissolved in 1% wt of aqueous solution of acetic acid, a novel chiral stationary phase based on sodium alginate coated onto silica gels has been successfully prepared. Fourier transforms infrared （F17R） spectroscopy and elemental analysis were used to characterize the chiral stationary phase. The chiral stationary phase was used for the separations of 10 racemates and 3 isomers with different ratios of hexane/isopropanol as the mobile phase. The experimental results showed that six chiral compounds （3- benzyloxy - 1,2-propanediol, DNB-leucine, 1 - （ 1-naphthyl）-ethanol, furoin, trans - 1,2-diphenyl oxide, alprenolol and two isomers o, m, p-hydroquinone and o, m, p-dibromobenzene were separated. This sodium alginate stationary phase possesses excellent enantioseparation ability.

关 键 词：海藻酸钠 手性固定相 手性分离 高效液相色谱 

分 类 号：O657.7[理学—分析化学]