红掌细菌性叶枯病病原菌的分离与鉴定  

Isolation and Characterization of Leaf Bacterial Blight Pathogen from Anthurium andraeanum

作　　者：梁丽雯[1,2] 任希望[1] 高志亮[1] 符秀梅[1] 林道哲[1] 罗红丽[1] 陈银华[1,2] 

机构地区：[1]海南大学海南省热带生物资源可持续利用重点实验室,海南海口570228 [2]海南大学农学院,海南海口570228

出　　处：《热带生物学报》2013年第4期327-331,共5页Journal of Tropical Biology

基　　金：国家自然基金(31060040;31260345);海南省重大科技专项(ZDZX2013023-02)

摘　　要：采用组织分离法,从发病的红掌叶片上分离、纯化病原菌,对纯化的病原菌进行菌落形态、致病性、生化特性测定,并对细菌特异基因ABC transporter进行分离及序列分析。结果表明,分离得到的病原菌为地毯草黄单胞菌属细菌,为红掌的致病菌。Bacterial leaf blight is a destructive disease of Anthurium spp. This disease, caused by Xanthomonas, Pseudomonas or Erwinia, had a major impact on the production of Anthurium industry in China. Pathogens were isolated from the leaves of Anthurium andraenum infected with Bacterial leaf blight at the Anthurium nursery in Hainan, China. The pathogens were purified for morphological observation, biochemical determination of patho- genic characteristics, and specific bacterial ABC transporter gene analysis. The results showed that the isolated pathogens were Xanthomonas axonopodis.

关 键 词：红掌 细菌性叶枯病 病原菌鉴定 地毯草黄单胞菌属(Xanthomonas axonopodis) 

分 类 号：S682.14[农业科学—观赏园艺]