蟒蛇肺炎克雷伯氏菌的分离与鉴定  

Isolation and Identification of Klebsiella pneumonia from the blood of a farmed Python molurus bivittatus infected with pneumonia

作　　者：郭桂英[1] 李祥[2] 黎丹[2] 廖承红[2] 杨诺[2] 曾纪峰 郑继平[2,3,4,5] 张立领 余集楠 

机构地区：[1]海南大学教务处,海南海口570228 [2]海南大学农学院,海南海口570228 [3]海南省热带生物资源可持续利用重点实验室 省部共建国家重点实验培育基地,海南海口570228 [4]海南大学热带生物资源教育部重点实验室,海南海口570228 [5]热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228 [6]海南大学材料与化工学院,海南海口570228

出　　处：《热带生物学报》2013年第4期332-334,346,共4页Journal of Tropical Biology

基　　金：海南省自然科学基金项目(313043);海南大学校青年基金(qnjj1147;qnjj1229);地方高校国家级大学生创新创业训练计划项目(201210589004)

摘　　要：亚洲岩蟒主要栖息在热带与亚热带森林,具有较高的药物、营养和经济价值,人工养殖中常因肺炎而死亡。为调查该病病因,对海南省养殖场发病蟒蛇进行心脏穿刺采集血样、细菌培养、革兰氏染色镜检、16S rRNA基因序列测定和BLAST分析等方法寻找病原,结果表明,分离菌株为肺炎克雷伯氏菌。Python molurus, as a large nonvenomous python species found in many tropical and subtropical for- ests, has been farmed for its high value in fine skin, nutrition and medicine in recent years. But pneumonia, the most serious and the most common disease of python, has caused death of python at a high rate. Blood sample was collected from a fanned Burmese python （Python molurus bivittatus） with pneumonia to determine the patho- gens of pneumonia. The specimens were incubated at 33 ℃ for 48 h to produce colony growth under the aerobic condition or with 5% CO2 in bacterial, actinomycetal and fungal mediums, separately. The isolated colonies were then characterized by gram staining and resorted to 16S rRNA gene sequencing and GenBank BLAST. The result showed that the isolates from the python was identified to be Klebsiella pneumonia.

关 键 词：蟒蛇 肺炎 肺炎克雷伯氏菌 

分 类 号：R378.991[医药卫生—病原生物学]