类转录激活因子效应物及其在生物技术领域的应用  

Transcription Activator Like Effectors and Its Application in Biotechnology

作　　者：吴伦英[1] 景晓辉[1] 沈雁[2,3] 

机构地区：[1]海南省热带作物资源可持续利用重点实验室 海南大学农学院,海南海口570228 [2]热带作物种质资源保护与开发利用教育部重点实验室 海南大学园艺园林学院,海南海口570228 [3]中国热带农业科学院椰子研究所,海南文昌571339

出　　处：《热带生物学报》2013年第4期386-392,共7页Journal of Tropical Biology

基　　金：海南省自然科学基金项目(809037;312039);海南省研究生创新科研课题(Hyb2012-1);国家公益性行业农业科研专项(200903049)

摘　　要：综述了类转录激活因子效应物(TALEs)技术的研究进展及生物技术应用前景,重点介绍TALEs的结构特征、作用机理、广谱抗性基因的人工构建及利用类转录激活因子效应物核酸酶(Transcription activator like effector nucleases,TALENs)进行基因组定点修饰的策略。Transcription activator like effectors （TALEs） are injected via the type Ⅲ secretion pathway of many plant pathogenic Xanthomonas spp. into plant cells where they contribute to infection of disease or trigger resist- ance by binding to DNA and turning on TALEs-specific host genes. All TALEs are composed of an N-terminal translocation domain, central tandem repeat DNA binding domain, C-terminal nuclear localization signals and an acidic transcriptional activation domain. Advances in our understanding of TALEs and their targets have yielded new models for pathogen recognition and defense. Recent elucidation of the basis for specificity in DNA binding by TALEs expedites further discovery and opens the door to biotechnological applications. Here, we summarized the recent advances and prospects of TALEs technology with an emphasis on its structure and functions, as well as the transcription activator-like effector nucleases.

关 键 词：类转录激活因子效应物 类转录激活因子效应物核酸酶 结构特征 生物技术应用前景 

分 类 号：Q814.9[生物学—生物工程]