铜、锌胁迫对珠芽蓼珠芽萌发及生理生化特性的影响  

Effects of Cu^(2+) and Zn^(2+) Stress on the Bulbil Germination and Physiological-biochemical Characteristics of Polygonum viviparum

作　　者：赵玉红[1] 蒙祖庆[1] 牛歆雨[1] 敬久旺[1] 魏学红[1] 

机构地区：[1]西藏大学农牧学院,西藏林芝860000

出　　处：《草地学报》2014年第1期116-121,共6页Acta Agrestia Sinica

基　　金：国家自然基金(31160112)(31240019);教育部青年基金(10XZJC850002)资助

摘　　要：为了探索珠芽蓼(Polygonum viviparum L.)修复铜、锌矿山土壤的机制,以珠芽蓼珠芽为材料,采用溶液培养试验研究了不同浓度的铜(Cu^(2+))和锌(Zn^(2+))胁迫对珠芽蓼珠芽萌发、生长及生理生化特性的影响。结果表明:Cu^(2+)浓度达到40 mg·L^(-1)时,发芽率下降;Zn^(2+)浓度达到20 mg·L^(-1)时,发芽率下降。不同浓度Cu^(2+)和Zn^(2+)与珠芽蓼幼苗高度和根长相关,Cu^(2+)对珠芽蓼珠芽幼苗生长和根系伸长的抑制作用比Zn^(2+)更显著。随着Cu^(2+)和Zn^2处理浓度的升高,珠芽蓼叶绿素a、叶绿素b含量降低;叶片电导率和丙二醛(MDA)含量增大,并与处理浓度呈一定的相关关系。珠芽蓼虽能够在西藏中部矿区正常生长而具有一定的耐性,但在铜、锌污染土壤的植物修复中没有较强的应用潜力。In order to understand the remediation mechanism of copper-zinc mine soil by Polygonum viviparurn, the effects of Cu and Zn stress on the bulbil germination rate, seedling growth and physiologi- cal-biochemical characteristics of Polygonurn viviparurn were investigated in this study. Results showed that the germination rate of buibil decreased when Cu2＋ concentration was more than 40 mg · L-1 or Zn2＋ more than 20 mg· L 1 The seedling height and root length of Polygonurn viviparum were correlated with the concentrations of Cu＋2 and Zn2＋. Cu2＋ had more significant effects on the seedling growth and root elongation of bulbil compared to Zn2＋. The chlorophyl1 a and chlorophyll b contents of bulbils de- creased with the increase of Cu2＋ or Zne＋ concentration; whereas the conductivity and malonaldehyde （MDA） contents of leaves increased. And all tested indexes were correlated with the concentrations of Cue＋ and Zne＋.

关 键 词：铜胁迫 锌胁迫 蓼珠芽 珠芽萌发 生理生化特性 POLYGONUM viviparum L 

分 类 号：Q945.78[生物学—植物学]