SRAP标记对8份假俭草材料的鉴定分析  

Identification Analysis of Eight Centipedegrass Materials by SRAP Molecular Markers

作　　者：郭海林[1] 郭爱桂[1] 宗俊勤[1] 袁学军[1] 张芳[1] 刘建秀[1] 

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园),江苏南京210014

出　　处：《草地学报》2014年第1期203-207,共5页Acta Agrestia Sinica

基　　金：江苏省科技支撑计划项目(BE2008403);江苏省平台项目(BM2010050);江苏省水利科技项目(2012053)资助

摘　　要：应用SRAP分子标记技术对6个假俭草（Eremochloa ophiuroides）优良品系和2个引进品种进行扩增扫描,从分子的角度对8份材料进行鉴定.结果表明：31对SRAP引物在8份材料中扩增出576条清晰的谱条,其中多态性条带359个,多态性比率为62.33％,8份材料间的遗传相似系数变异范围为0.6962～0.8177,平均遗传相似系数为0.7388.31对SRAP引物中有1对引物Me18Em17可以将所有材料区分开来,应用该引物的扩增谱带构建了参试材料的DNA指纹图谱,可将不同材料从DNA水平上加以区分.Six excellent strains and two cultivars of centipederass were amplified by 31 SRAP primers. As a result, a total of 576 clear bands were detected and 359 were polymorphic with PPB （Percentage of Poly morphic Bands） at 62. 33%. The genetic similarity among eight tested materials ranged from 0. 6962 toO. 8177 with the average of 0. 7388. One primer of Me18Em17 was selected to construct DNA fingerprint of 8 tested materials. It was able to distinguish each tested cultivar from others by polymorphic bands.

关 键 词：假俭草 优良品系 鉴定 

分 类 号：S331[农业科学—作物遗传育种]