低温胁迫下冬小麦东农冬麦1号分蘖节相关EST-SSR信息分析  

Analysis of EST- SSR information in tillering nodes of winter wheat Dongnongdongmai1 under low temperature stress

作　　者：苍晶[1] 范博[1] 宋扬[1] 许贺[1] 牟永潮[1] 于晶[1] 张达[1] 王军虹[1] 孟婧[1] 朱祥春[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2014年第1期1-5,共5页Journal of Northeast Agricultural University

基　　金：高等学校博士学科点专项科研基金课题(20112325110003);黑龙江省教育厅科学技术研究项目;黑龙江省教育厅青年基金项目(11551067)

摘　　要：利用抑制消减杂交技术构建东农冬麦1号分蘖节低温胁迫相关基因的cDNA正反文库,获得表达序列标签EST(Expressed sequence tag),经分析和前期处理后拼接得到736条非重复序列(Unigenes)。运用生物信息学方法对低温胁迫下诱导的冬小麦东农冬麦1号分蘖节相关EST-SSRs分布频率和碱基重复特点进行归纳分析。结果表明,736条Unigenes共检索到59个EST-SSRs,EST-SSRs出现频率为8.02%。EST-SSRs包括24种重复单元,其主要以三核苷酸和二核苷酸重复基元为主,二者分别占总SSRs 47.46%和44.07%,在EST中出现频率分别为3.80%和3.53%,其中以二核苷酸CA和AG重复基元为主,三核苷酸重复基元以AAC重复基元所占比例最高。为冬小麦EST资源开发和抗寒基因挖掘奠定基础。Expressed sequence tags （EST） were obtaineded from positive and negative cDNA library of low temperature stress related genes in Dongnongdongmail,which was constructed by suppression subtractive hybridization technique. After analysis and pretreatment, the EST were assembled and 736 unigenes were acquired. The distribution frequency and the characteristics of base-repeat of EST-SSRs in tillering nodes of Dongnongdongmail under low temperature were summarized and analyzed using the bioinformatics method. The results showed that All of 56 EST-SSRs distributed in 736 unigenes. The frequency of EST-SSRs occurences was 8.02%. These EST-SSRs were composed of 24 kinds repeat motifs and they were basically trinucleotide or dinucleotide repeat motifs. In the total SSRs, the trinucleotide repeat motifs accounted for 47.46% and the dinucleotide repeat motifs accounted for 44.07%.1n the total EST frequency, the trinucleotide repeat motifs accounted for 3.80% and the the dinucleotide repeat motifs accounted for 3.53%. The repeat motif on CA and AG in dinucleotide repeat motifs were the most common motif, and The repeat motif on AAC in trinucleotide repeat motifs accounted for the greatest proportion. All these conclusions will establish the theoretic foundation for development EST resource of winter wheat and cold resistance gene excavation.

关 键 词：冬小麦 东农冬麦1号 低温胁迫 分蘖节 EST SSR 

分 类 号：S512.11[农业科学—作物学]