水稻Os03g19020基因的克隆及亚细胞定位分析  

Cloning and subcellular localization analysis of Os03g19020 gene in rice

作　　者：柏锡[1] 贾蓓[1] 吕德康[1] 朱延明[1] 才华[1] 纪巍[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2014年第1期29-33,共5页Journal of Northeast Agricultural University

基　　金：黑龙江省高校科技创新团队建设计划(2011TD005);东北农业大学博士启动基金(2009RC06)

摘　　要：Os03g19020基因是从水稻苗期冷害基因表达谱芯片中筛选到一个冷胁迫诱导表达的基因,Real-time PCR验证。结果表明,Os03g19020基因受冷诱导表达,响应迅速;氨基酸序列分析发现Os03g19020编码PHDfinger家族蛋白,具有典型的C4HC3保守结构域;同源性分析结果显示,03g19020与籼稻品种OsI_11202蛋白相似性达98%。将03g19020基因融合GFP基因,转化烟草原生质体细胞,结果表明,03g19020蛋白主要定位在细胞核内。Os03919020 was identified as a cold-responsive gene based on genome profiles and Real-time PCR analysis in response to cold stress. The Os03g19020 mRNA showed rapid up-regulation following cold-stress treatment. The amino acid sequence analysis revealed that Os03g19020 encoding PHD-finger family proteins, with a typical C4HC3 conserved domain; homology analysis showed that 03g19020 was similar to indica rice OsI_11202 protein, and the serial similarity was about 98%. The subcellular localization mainly in the nucleus suggested that Os03g19020 was an transcription factor.

关 键 词：水稻 Os03g19020基因 PHD—finger 亚细胞定位 

分 类 号：S572[农业科学—烟草工业]