ISSR和SSR体系优化及在分析不同核桃品种遗传多样性上的应用  

ISSR and SSR system optimization and application on genetic diversity analyzing of walnut

作　　者：翟梅枝[1] 肖志娟[1] 许静[1] 孙宇栋[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《东北农业大学学报》2014年第1期90-97,共8页Journal of Northeast Agricultural University

基　　金：公益性行业科研专项(201004027);陕西省科技统筹创新工程计划(2012KTZB02-01)

摘　　要：对核桃叶总DNA进行提取，筛选出多态性强重复性好的15条ISSR引物和12对SSR引物。利用ISSR和SSR分子标记技术对不同核桃品种遗传多样性进行研究。结果表明，15条ISSR引物共检测到187个条带，其中132个呈多态性，多态性百分比为70．59％，平均每条引物扩增出多态性位点8．8个；不同品种间相似系数变化范围为0．2500-0．7639，多态信息含量（PIC）变化范围在0．7105～0．9151，其值均大于0．5；12对SSR共检测到174个条带，其中118条呈现态性，多态性百分比为67．82％，平均每条引物扩增出多态性位点9．8个，遗传相似系数变化范围为0．1594～0．8036，多态信息含量（PIC）变化范围在0．7422～0．8962，平均值为0．8150，其值均大于0．5。用Mantel检测对两种标记结果进行相关性分析（r=0．6257，P=I），表明本研究中ISSR标记和SSR标记的相关性显著。基于ISSR和SSR综合后数据遗传相似性系数的UPGMA聚类结果表明，在遗传相似系数阈值0．39，可将供试材料分成四大类。研究结果显示，供试材料遗传资源丰富，可作为育种来源。In this study, ISSR and SSR molecular marker technologies were selected to analyze the genetic diversity of different walnut varieties to provide a theoretical basis for the future development of new varieties and germplasm innovation. Total 15 ISSR primers and 12 SSR primers with good repeatability and strong polymorphism were screened and the PCR optimum system was set up. ISSR molecular mark detected 187 bands, of which 132 were polymorphism bands, accounting for 70.59%, and the number of the polymorphic loci of each primer averaged 8.8. The similarity coefficient ranged from 0.2500 to 0.7639. Polymorphic information content （PIC） ranged from 0.7105 to 0.9151, and each value was greater than 0.5. SSR molecular mark detected 174 bands, of which 118 were polymorphism bands, accounting for 67.82%, and the number of the polymorphic loci of each primer averaged 9.8.The similarity coefficient ranged from 0.1594 to 0.8036. Polymorphic information content （PIC） ranged from 0.7422 to 0.8962, and each value was greater than 0.5. Mantel test was used to analyse correlation of ISSR and SSR （r=0.6257, P=1）, indicated that ISSR markers and SSR markers in this study was consistent. Cluster analysis with UPGMA method showed that the samples could be divided into four groups. It showed that the genetic resources of these samples were very rich, and could be used as breeding source.

关 键 词：核桃 ISSR SSR 体系优化 遗传多样性 

分 类 号：S664.1[农业科学—果树学]