上海某医院2011年1至5月住院病例2044份血及体液标本血培养结果分析  

Analysis on the blood culture results of 2 044 blood and body fluid specimens of inpatients from a hospital in Shanghai from January to May,2011

作　　者：赵忆文 朱召芹[2] 蔡金凤[2] 陈海丽[2] 王茉婴[2] 胡芸文[2] 张军[2] 宰淑蓓[2] 

机构地区：[1]普陀区妇婴保健院,上海200060 [2]上海市公共卫生临床中心,上海201508

出　　处：《检验医学》2014年第2期100-103,共4页Laboratory Medicine

基　　金：国家自然科学基金青年基金项目(31200108);十二五科技重大专项(2013ZX10004-221和2013ZX10004221-004)

摘　　要：目的监测和分析上海市公共卫生临床中心血及体液标本血培养分离各种细菌的分布趋势,评价全自动血培养仪阳性结果中的菌群类型和假阳性/假阴性情况。方法采用法国生物梅里埃公司全自动血培养仪和细菌鉴定仪对2011年1至5月收集的2 044份血及体液标本进行培养和鉴定,并进行结果分析。结果不同标本的细菌阳性检出率不同,穿刺液标本的细菌阳性检出率最高(38.10%),其次为血液标本(30.74%)。共分离出231株细菌,总阳性检出率为11.30%,均为单一菌种。其中凝固酶阴性葡萄球菌最多(41.13%),其次为真菌(18.18%),金黄色葡萄球菌所占比例最少(0.87%)。结论了解血及体液标本培养的菌群类型和分布情况,对致病菌和污染菌进行综合鉴别和分析,将有助于指导或帮助临床用药,预防败血症的发生。Objective To analyze and monitor the distribution trend of bacterium strains in blood cultures for blood and body fluid specimens in Shanghai Public Health Clinical Center,and to evaluate the false positive /false negative detection results of automated blood culture analyzer.Methods A total of 2 044 blood culture results of different clinical blood and body fluid specimens from January to May,201 1 were collected.Specimens were cultured and identified by M＆#233;li＆#232;s automated blood culture analyzer and identification board.The results were analyzed.Results Different specimens had different positive rates.The puncture fluid specimens had the highest bacterium culture positive rate （38.1 0%）,followed by blood specimens （30.74%）.The 231 bacterium strains were isolated.The total positive rate was 1 1 .30%.Coagulase-negative Staphylococcus was the most,accounting for 41 .1 3%,followed by fungi （1 8.1 8%）and Staphylococcus aureus （0.87%）.Conclusions The bacterium identification and distribution of the blood and body fluid specimens and the analysis of pathogens and contaminative bacteria will help for guiding the clinical treatment and prevention of sepsis.

关 键 词：血培养 细菌分离和提纯 全自动血培养仪 

分 类 号：R446.5[医药卫生—诊断学]