血清ox-LDL与非酒精性脂肪肝超声检查关系的探讨  

Investigation on the correlation of serum ox-LDL level with ultrasound image for nonalcoholic fatty liver disease

作　　者：胡可胜[1] 马艳华[1] 刘连红[1] 李伟[2] 罗建祥[1] 

机构地区：[1]武警广东总队医院检验科,广东广州510000 [2]武警广东总队医院消化科,广东广州510000

出　　处：《检验医学》2014年第2期118-121,共4页Laboratory Medicine

摘　　要：目的探讨不同程度非酒精性脂肪肝(NAFLD)患者血清氧化低密度脂蛋白(ox-LDL)水平及其与代谢指标的相关性。方法采用酶联免疫吸附试验(ELISA)检测79例NAFLD患者和23名正常对照者血清oxLDL浓度,同时测定肝功能、代谢指标及血清同型半胱氨酸(Hcy)水平,根据B超检查结果将79例NAFLD患者分为轻(40例)、中(25例)、重度(14例)脂肪肝。分析ox-LDL与其他指标及脂肪肝程度的相关性。结果 NAFLD组丙氨酸氨基转移酶、γ谷氨酰基转移酶、甘油三酯、总胆固醇、低密度脂蛋白胆固醇、载脂蛋白B、载脂蛋白E、Hcy、尿酸、空腹血糖、纤维连接蛋白及ox-LDL水平均明显高于正常对照组(P均<0.05)。中、重度脂肪肝组血清ox-LDL水平明显高于轻度脂肪肝组(P均<0.05)。ox-LDL与Hcy、尿酸、总胆汁酸呈正相关关系[相关系数(r)为0.375、0.369和0.395,P值分别为0.007、0.013和0.029],ox-LDL水平与脂肪肝的分级呈正相关(r=0.410,P=0.011)。结论随着脂肪肝程度的加重,NAFLD患者血清ox-LDL水平逐渐升高。ox-LDL是肝功能受损及脂肪肝进展过程中的重要因素,对临床诊断和病情监测有重要意义。Objective To investigate the correlation of serum oxidized-low density lipoprotein （ox-LDL）with metabolism parameters in different graded nonalcoholic fatty liver disease（NAFLD）patients.Methods Enzyme-linked immunosorbert assay （ELISA）was used to determine the levels of serum ox-LDL of 79 NAFLD patients and 23 healthy controls, and the liver functions,metabolism parameters and serum homocysteine （Hcy ） were determined simultaneously.By ultrasound image as the diagnostic standard,NAFLD patients were classified into mild （40 cases）, moderate （25 cases）and severe （1 4 cases）fatty liver groups.The correlation of ox-LDL with other parameters and fatty liver infiltration grade was analyzed.Results Alanine aminotransferase,gamma-glutamyltransferase,triglyceride,total cholesterol,low-density lipoprotein cholesterol,apolipoprotein B,apolipoprotein E,Hcy,uric acid,fasting blood glucose,fibronectin and ox-LDL were significantly higher in NAFLD group than in healthy control group（P〈0.05）. Serum ox-LDL levels in mild fatty liver group were significantly lower than those in moderate fatty liver group and severe fatty liver group （P〈0.05 ）.Serum ox-LDL was positively correlated with Hcy,uric acid and total bile acid levels [correlation coefficients （r）=0.375,0.369 and 0.395;P=0.007,0.01 3 and 0.029],and was positively correlated with fatty liver infiltration grade （r=0.41 0,P=0.01 1 ）.Conclusions The serum ox-LDL level in NAFLD patients increases with the increasing of fatty liver infiltration grade,and ox-LDL is an important factor in liver function impairment and fatty liver disease progression.

关 键 词：氧化低密度脂蛋白 脂肪肝程度 同型半胱氨酸非 酒精性脂肪肝 

分 类 号：R446.1[医药卫生—诊断学]