高同型半胱氨酸血症患者氧化应激指标的研究  

Research on the parameters of oxidative stress in patients with hyperhomocysteinemia

作　　者：蒋兴亮[1] 刘素兰[1] 易婷婷[1] 

机构地区：[1]川北医学院附属医院检验科,四川南充637000

出　　处：《检验医学》2014年第2期125-129,共5页Laboratory Medicine

基　　金：四川省卫生厅资助项目(20090142)

摘　　要：目的观察高同型半胱氨酸血症(HHcy)患者氧化应激指标的水平,并对其临床价值做初步评价。方法检测108例HHcy患者、106名健康体检者(正常对照组)循环谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、对氧磷酯酶1(PON1)、一氧化氮合酶(NOS)活性及同型半胱氨酸(Hcy)、一氧化氮(NO)和丙二醛(MDA)水平。分析Hcy与GSH-Px、SOD、PON1、NOS、NO、MDA之间的相关性。结果 HHcy患者血浆MDA水平[(6.23±1.55)μmol/L]明显高于正常对照组[(4.14±1.13)μmol/L](P<0.01),而GSH-Px[(189.3±25.1)U/L]、SOD[(77.3±20.5)NU/mL]、PON1[(133.6±23.9)kU/L]、NOS活性[(25.3±2.9)U/mL]及NO[(68.3±10.1)μmol/L]水平低于正常对照组[(240.3±78.1)U/L、(89.2±24.8)NU/mL、(168.2±26.0)kU/L、(30.0±3.3)U/mL、(92.1±12.1)μmol/L](P均<0.01)。HHcy患者血浆Hcy与MDA呈正相关(r=0.72,P<0.01),与GSH-Px、SOD、PON1、NOS、NO呈明显负相关(r值分别为-0.60、-0.49、-0.51、-0.43、-0.50,P均<0.01)。结论 HHcy患者氧化应激增强可能与Hcy氧化过程中产生过多的过氧化物及活性氧、Hcy损伤NO/L-精氨酸系统及直接抵制抗氧化酶活性有关。Hcy可能通过增加氧化应激和降低抗氧化能力在动脉粥样硬化发生、发展中起重要的作用。Objective To observe the parameters of oxidative stress in patients with hyperhomocysteinemia （HHcy）,and to evaluate the clinical significance primarily.Methods A total of 1 08 HHcy patients and 1 06 healthy subjects （healthy control group ） were enrolled for determining the activities of glutathione peroxidase （GSH-Px ）, superoxide dismutase （SOD）,paraoxonase 1 （PON1 ）and nitric oxide synthase （NOS）and the levels of homocysteine （Hcy）,nitric oxide （NO）and malondialdehyde （MDA）.The correlations of Hcy with GSH-Px,SOD,PON1 ,NOS, NO and MDA were analyzed.Results The MDA level in patients with HHcy [（6.23 ±1 .55）μmol/L]was higher than that in healthy control group [（4.1 4 ±1 .1 3）μmol/L]（P〈0.01 ）.The GSH-Px [（1 89.3 ±25.1 ）U/L],SOD [（77.3 ±20.5）NU/mL],PON1 [（133.6 ±23.9）kU/L],NOS [（25.3 ±2.9）U/mL]and NO [（68.3 ± 1 0.1 ）μmol/L]in patients with HHcy were lower than those in healthy control group [（240.3 ±78.1 ）U/L,（89.2 ± 24.8）NU/mL,（168.2 ±26.0）kU/L,（30.0 ±3.3）U/mL and （92.1 ±12.1 ）μmol/L]（P〈0.01 ）.The correlation of Hcy with MDA was positive,and negative with GSH-Px,SOD,PON1 ,NOS and NO （r =-0.60,-0.49,-0.51 ,-0.43 and -0.50,P〈0.01 ）.Conclusions Oxidative stress in patients with HHcy is related with pro-oxidant and antioxidant imbalance,Hcy impairment NO/L-arginine system and antioxidase activity defense systems. Hcy plays a role in the pathogenesis and development of atherosclerosis by increasing the oxidative stress and decreasing the ability of antioxidation.

关 键 词：氧化应激 高同型半胱氨酸血症 对氧磷酯酶1 PARAOXONASE 1 

分 类 号：R446.1[医药卫生—诊断学]