利用TAIL-PCR技术克隆拟南芥抗旱相关的csm2基因  

Cloning of an Arabidopsis drought-tolerant csm2 gene by using TAIL-PCR technology

作　　者：程丹[1] 陈光朗[1] 孙成磊[1] 阳立波 曹树青[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《合肥工业大学学报（自然科学版）》2014年第2期229-231,共3页Journal of Hefei University of Technology：Natural Science

基　　金：高等学校博士学科点专项科研基金资助项目(20120111110009);中国博士后基金资助项目(2012M521213);安徽省青年科学基金资助项目(1308085QC56)

摘　　要：文章在400mmol/L甘露醇的筛选条件下,获得一个T-DNA插入的拟南芥抗旱突变体,命名为csm2-1。采用TAIL-PCR技术获得了T-DNA插入位点的侧翼序列,序列分析证实T-DNA插入基因为At1g07530,该基因编码植物体内的一类GRAS家族的转录调控因子。该研究为进一步阐明GRAS家族基因参与干旱胁迫的分子机制提供新的思路,同时也为植物抗逆分子育种提供候选基因。A drought-sensitive mutant ,named csm2-1 ,was isolated by using 400 mmol/L mannitol treatment .The csm2 gene was cloned by using TAIL-PCR technology ,and the T-DNA insertion site was found to be located at At1g07530 ,which was shown to encode one member of GRAS family tran-scription factors in plants .The results obtained provide not only new ideas for the description of mo-lecular mechanism of GRAS family genes’ participation in drought stress but also a candidate gene for drought-tolerant molecular breeding .

关 键 词：抗旱 鉴定 基因克隆 拟南芥 

分 类 号：Q754[生物学—分子生物学]