三氮脒在猪血浆的药物-蛋白结合研究  

Drug-protein binding of diminazene in swine plasma

作　　者：李博[1] 赵吉祥[1] 陈杖榴[1] 路文华 韩可可 徐维[1] 陈红[1] 

机构地区：[1]华南农业大学兽医学院/国家兽药残留基准实验室,广东广州510642

出　　处：《华南农业大学学报》2014年第2期24-27,共4页Journal of South China Agricultural University

基　　金：2009年农业行业标准制定"动物性食品中三氮脒残留量的测定"(农财发[2009]105号)

摘　　要：[目的]研究三氮脒临床用药的有效性和安全性，测定猪血浆的药物-蛋白结合率．[方法]血浆样品以超滤法分离游离型的药物．以高效液相色谱-紫外法测定药物含量，计算出三氮脒-血浆蛋白结合率．6头猪交叉分别按体质量肌内注射7和14 mg/kg三氮脒注射液，在给药后3、5、15、60 min测定体内三氮脒-血浆蛋白结合率．[结果和结论]体外测定的三氮脒-血浆药物蛋白结合率为74.68％～95.90％．猪给药后体内的药物蛋白结合率为84.11％～89.69％（7 mg/kg剂量组）、82.96％～88.65％（14 mg/kg剂量组），随着剂量增加可能出现饱和现象．在设计三氮脒应用于猪的给药方案时应充分考虑这一因素．[Objective] A study on the diminazene-protein binding rate in swine was carried out in this ex-periment for further clinical application efficiency and safety .[Method] The plasma samples were pretrea-ted to separate free drug by ultrafiltration .A high performance liquid chromatographic-UV （ HPLC-UV） method was used to measure the amount of free diminazene in plasma .Then the drug-protein binding rate was accounted .Six swine were administrated intramuscularly at a dosages of 7 and 14 mg/kg.The plas-ma samples were collected and the protein binding rates in vivo were determination at 3, 5, 15, 60 min.[Result and conclusion] Diminazene-protein binding rate tested in vitro was within the range of 74.68%-95.90%.Although the drug-protein binding rate in vivo was within the range of 84.11% -89.69%（7 mg/kg, i.m.）, and 82.96%-88.65%（14 mg/kg, i.m.） after administration, and saturation phe-nomenon emerged as dosage increased .These factors should be considered in clinical regimens designing .

关 键 词：三氮脒 药物-蛋白结合 猪血浆 

分 类 号：S859.79[农业科学—临床兽医学]