枸杞子糖缀合物及其糖链对LDL氧化修饰的抑制作用  

STUDIES ON THE GLYCOCONJUGATES AND GLYCANS FROM LYCIUM BARBARUM L IN INHIBITING LOW DENSITY LIPOPROTEIN (LDL) PEROXIDATION

作　　者：黄琳娟[1] 田庚元[1] 王仲孚[1] 董继斌[2] 吴满平[2] 

机构地区：[1]中国科学院上海有机化学研究所生命有机化学国家重点实验室,上海200032 [2]上海医科大学药学院,上海200031

出　　处：《药学学报》2001年第2期108-111,共4页Acta Pharmaceutica Sinica

基　　金：国家攀登计划!(970 2 110 0 6 4 4)资助项目

摘　　要：目的　研究枸杞子糖缀合物及其糖链对LDL氧化修饰的抑制作用。方法　在Cu2 +诱导的LDL氧化模型中测定了脂质过氧化产物硫代巴比妥酸反应物质 (TBARS)的含量及LDL在琼脂糖凝胶电泳上的迁移率以反映从枸杞子中分离、纯化得到的糖缀合物及糖链对LDL氧化修饰的抑制作用。结果　枸杞子糖缀合物及糖链抗LDL氧化的能力是不同的 ,其中LbGp5可明显的抑制LDL的氧化。结论　枸杞子糖缀合物具有抗LDL氧化作用 。AIM To determine the effects of glycoconjugates and their glycans from Lycium barbarum L. on inhibiting low density lipoprotein (LDL) peroxidation. METHODS Using Cu 2+ induced oxidation as a model, the oxidative production of thiobarbituric acid reactive substances (TBARS) and the LDL electrophoresis migration on agarose gel were measured. RESULTS The effects of glycoconjugates and their glycans from Lycium barbarum L. on inhibiting LDL peroxidation were different, among them, glycoconjugate LbGp5 showed the best effect on inhibiting LDLperoxidation. CONCLUSION The glycoconjugates can inhibit LDL peroxidatin while their glycans showed no effects on inhibiting LDL peroxidation.

关 键 词：枸杞子糖缀合物 低密度脂蛋白 氧化修饰 糖链 动脉粥样硬化 

分 类 号：R543.505[医药卫生—心血管疾病]