紫杉醇抑制血管生成的研究  

Inhibitory Effect of Paclitaxel on Angiogenesis

作　　者：阎家麒 王悦[2] 

机构地区：[1]河南绿十字生物工程研究所,郑州450008 [2]中山医科大学肿瘤防治中心,广州510060

出　　处：《药物生物技术》2001年第1期30-32,共3页Pharmaceutical Biotechnology

摘　　要：采用大鼠动脉环无血清培养形成微血管方法 ,测定紫杉醇抑制血管生成作用。将大鼠动脉段包埋于纤维蛋白胶中 ,用MCDB131无血清培养液进行体外培养 ,内皮细胞从动脉段切口处向外生长 ,形成具有分枝的微血管样结构。每日计数血管生成量 ,绘制生长曲线。结果表明 ,紫杉醇对血管生成有很强的抑制效应 ,证实紫杉醇是一种血管生成抑制因子。动脉环模型是一种有用的血管生成动态、定量的体外测定方法。The inhibitory effect of paclitaxel on angiogenesis was measured by the experiment of the growth of microvessels in serum free matrix culture of rat aorta. The rings of rat aorta was embedded in gel of fibrin and cultured in MCDB131 in vitro . The microvessels originated from the ends of resection of the aorta, and generated branching microvessels, curves of microvascular growth were generated by counting the number of microvessels daily. The results showed that paclitaxel has inhibitory effect on angiogenesis strongly, it is a specific angiogenesis angiogenesis inhibiting factor. Our results demonstrate that the aortic ring model can be used as a dynamic and quantitative assay for the study of angiogenesis in vitro .

关 键 词：紫杉醇 血管生成 动脉环无血清培养 抑制作用 抗癌药 

分 类 号：R979.1[医药卫生—药品]