不同浓度Neogenin对滋养细胞侵袭能力改变的影响研究  

Study on the effect of different concentrations of neogenin on change of invasive ability of trophoblasts

作　　者：钟少平[1] 彭忠英[1] 刘霞[1] 沈华祥[1] 曹云飞[1] 彭华[1] 冯英[1] 

机构地区：[1]浙江省嘉兴市妇幼保健院,314051

出　　处：《中国妇幼保健》2014年第7期1089-1091,共3页Maternal and Child Health Care of China

基　　金：浙江省医药卫生科技计划项目〔2012KYB209〕;嘉兴市科技局基金资助项目〔2012AY1073-1;2012AY1073-5;2012AY1073-6〕;嘉兴市政府重点科技创新团队基金〔2011年度生殖与遗传创新团队专项基金〕

摘　　要：目的：探讨不同浓度Neogenin对滋养细胞侵袭能力改变的影响。方法：行早孕期滋养细胞株TEV-1培养，并随机分为以无血清培养基处理的对照组及含不同浓度Neogenin（1～50ng／m1）的无血清培养基处理的实验组，培养24h后利用细胞免疫荧光法行滋养细胞MMP-9定位检测，采用改良Boyden小室法监测不同浓度Neogenin刺激后滋养细胞侵袭能力的变化。利用蛋白免疫印迹技术检测不同浓度Neogenin刺激后滋养细胞MMP9蛋白表达变化情况。结果：MMP-9表达于滋养细胞胞浆。在培养的滋养细胞中加入不同剂量的Neogenin干预滋养细胞24h后，滋养细胞MMP-9表达量均下降，当Neogenin的干预剂量达到5ng／ml时，穿膜成功细胞数明显减少（P〈0．05）。结论：Neogenin可能通过影响TEV-1细胞MMP-9活性的改变而导致其侵袭能力变化。Objective： To explore the effect of different concentrations of neogenin on change of invasive ability of trophoblasts. Methods ： TEV - 1 cell line was cultured and subcuhured in medium without serum, then the cells were treated with different concentrations of neogenin （1 -50 ng/ml） as experimental groups, while the cells not treated with neogenin were selected as control group, after 24 hours, the position of matrix metalloproteinase - 9 （ MMP - 9 ） were detected with cell immunofluorescence. The change of invasion ability of tropho- blasts was monitored by modified Boyden small chamber. The expression of MMP - 9 was detected by western blotting. Results ： MMP - 9 located in cytoplasm of trophoblasts. The expression of MMP - 9 decreased with the intervention of different concentrations of neogenin for 24 hours, when the dosages of neogenin increased to 5 ng/ml, the number of transmission ceils decreased significantly （P 〈 0. 05 ） . Conclu- sion ： Neogenin may induce the change of invasive ability of TEV - 1 ceils by affecting the change of activity of MMP - 9 in TEV - 1 cells.

关 键 词：Neogenin滋养细胞侵袭 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]