固相萃取净化-高效液相色谱测定水果中赤霉素、多效唑、烯效唑残留量  

Determination of gibberellin,paclobutrazol and uniconazole residues in fruits by SPE-HPLC

作　　者：张敏[1] 闫超杰 付海滨[3] 李修平[4] 徐宜宏[3] 

机构地区：[1]沈阳农业大学工程学院,辽宁沈阳110866 [2]锦州出入境检验检疫局,辽宁锦州121013 [3]沈阳出入境检验检疫局,辽宁沈阳110016 [4]济宁出入境检验检疫局,山东济宁272100

出　　处：《食品与发酵工业》2014年第2期192-195,共4页Food and Fermentation Industries

基　　金：沈阳市2012年科学技术计划农业攻关项目(No.F10-235-4-00)

摘　　要：建立了一种简单、快速，同时检测水果中赤霉素、多效唑和烯效唑残留量的固相萃取-高效液相色谱（SPE—HPLC）方法。该方法经80％甲醇水提取样品，C18固相萃取小柱净化，采用DAD检测器220nm紫外扫描检测。结果表明：3种植物生长调节剂在0．5～10μg／mL内线性关系良好（r≥0．999）；检出限分别为赤霉素0．002mg／kg、多效唑0．04mg／kg、烯效唑0．02mg／kg；在0．2～0．8mg／kg加标水平下，3种植物生长调节剂平均回收率为78％～91％；相对标准偏差为2．4％～5．4％。A simple and rapid SPE-HPLC method for the determination of Gibberellin, Paclobutrazol and Unicon- azole residues in fruits was developed. The sample was extracted with 80% methanol -water solution, then was puri- fied by Cls column and detected in 220 nm with a DAD detector. Linear ranges of three plant growth regulators were in the range of 0.5 - 10 μg/mL with correlation coefficients more than 0. 999. The minimum detection limit of Gib- berellin, Paclobutrazol and Uniconazole were 0. 002 mg/kg, 0.04 mg/kg and 0.02 mg/kg respectively. The average recoveries at s tions （RSDs） piked concentration levels of 0.2 -0.8 mg/kg ranged from 78% to 91% , with relative standard devia- of 2.4% -5.4%.

关 键 词：固相萃取 高效液相色谱 水果 赤霉素 多效唑 烯效唑 残留 

分 类 号：O657.72[理学—分析化学]