高温对转基因地被菊的影响  

Influence of High Temperature on Ground Cover Chrysanthemum

作　　者：熊毅[1] 郭彦萃[1] 张志[1] 

机构地区：[1]齐齐哈尔大学,齐齐哈尔161006

出　　处：《东北林业大学学报》2014年第3期45-47,共3页Journal of Northeast Forestry University

基　　金：黑龙江省教育厅科学技术研究项目(1551538)

摘　　要：以已转DREB1A基因地被菊的4个株系为研究对象,以野生型植株为对照,分析了其在高温胁迫条件下形态及生理指标变化规律。结果表明:经48 h高温胁迫后有3个转基因株系的叶片相对含水量显著高于野生型植株,电导率及MDA(丙二醛)质量摩尔浓度均以野生型最高;当胁迫时间大于24 h时,转基因株系的SOD(超氧化物歧化酶)活性均高于野生型;在各胁迫时间点内野生型植株POD(过氧化物酶)活性均低于转基因株系;形态指标方面,QY2、QY3的株高、冠幅胁迫指数均显著高于野生型。从而得出转基因株系与野生型植株相比具有一定的高温胁迫耐性。Four transgenic lines of ground cover chrysanthemum with over-expressing DREB1A that conferred strong tolerance of drought stress were treated under high temperature with the ＇ Wild Type＇ as check. After 48 h stress, there were three transgenic lines significant higher than ＇ Wild Type＇ RWC content, and ＇ Wild Type＇ was highest in MDA content. When stress time was longer than 24 h, SOD and POD content in ＇ Wild Type＇ was the lowest. The PHSI index in QY2 and QY3 lines were also significant higher than ＇ Wild Type＇, Therefore, the transgenic lines have higher temperature tolerance than check.

关 键 词：地被菊 高温胁迫 抗旱基因 

分 类 号：S682.11[农业科学—观赏园艺] Q945.78[农业科学—园艺学]