桑氏链霉菌产几丁质酶特性及对杨树紫纹病的生防作用  

Characteristics of Chitinase-Produced by Streptomycete sampsonii with Antimicrobial Activity and Its Biocontrol to Rhizoctonia violacea

作　　者：李姝江[1] 朱天辉[1] 彭艳[1] 雷美艳[1] 韩珊[1] 

机构地区：[1]四川农业大学,雅安625014

出　　处：《东北林业大学学报》2014年第3期116-121,共6页Journal of Northeast Forestry University

基　　金：国家自然科技资源共享平台项目(2005DKA21207-13)

摘　　要：以紫丝核菌(Rhizoctonia violacea)为靶生物,通过对峙培养法从健康的杨树根际土壤中筛选到一株对其有较好拮抗效果的产几丁质酶的菌株112903。根据形态特征、培养特性及生理生化特性和16 S rDNA序列分析结果,将菌株112903定名为桑氏链霉菌(Streptomyces sampsonii 112903)。通过单因素试验和正交试验进行发酵条件优化,获得S.sampsonii 112903最佳产几丁质酶条件为:胶体几丁质300 mL/L,蛋白胨0.4%,K2HPO41.0 g/L,KH2PO40.6 g/L,初始pH值7.0,培养温度26℃,培养时间144 h。田间试验表明:优化培养的发酵滤液可降低杨树紫纹羽病发生率,且防效显著。With Rhizoctonia violacea as the target creature, and chitinase-producing strain 112903 which had a better antagonistic effect was screened from the rhizospheric soil of healthy poplar by the confront culture method. According to the morphological, cultural and physio-biochemical characteristics and 16S rDNA sequence alignment, the strain 112903 was identified as Streptomyces sampsonii. By single factor and orthogonal test, the optimal chitinase production conditions of S. sampsonii 112903 were 300 mL/L of colloidal chitin, 0.4% of peptone, 1.0 g/L of K2HPO4, 0.6 g/L of KH2PO4, 7.0 of initial pH, 26℃ and 144 h. By the experiments in the field, the fermentation filtrate optimized can reduce morbidity rate of poplar purple root rot, and the control efficacy is significant.

关 键 词：杨树紫纹羽病菌 链霉菌 几丁质酶 生物防治 

分 类 号：S763.1[农业科学—森林保护学]