辣椒经地面模拟诱变后的RAPD鉴定  

The RAPD Detection of Capsicum annuum L. After Ground Simulation

作　　者：郭亚华[1] 谢立波[1] 王雪[1] 陈立新[1] 刘录祥[2] 周宇[1] 

机构地区：[1]黑龙江省农业科学院园艺分院,黑龙江哈尔滨150069 [2]中国农业科学院作物科学研究所,国家农作物航天诱变技术改良中心,北京100081

出　　处：《北方园艺》2014年第5期95-97,共3页Northern Horticulture

基　　金：国家"863"计划资助项目(2012AA101202);国家大宗蔬菜产业技术体系哈尔滨综合试验站资助项目(CARS-25-G-11)

摘　　要：以地面模拟处理辣椒干种子为试材,采用RAPD技术对辣椒染色体进行多态性检测,研究了不同剂量的物理诱变处理对辣椒产生的影响。结果表明:采用Li离子照射可以诱导辣椒发生遗传位点的改变,但是不同剂量的效应不同;采用物理进行诱变可以诱导多个性状发生改变,该方法可以产生复杂的遗传变异。Taking the seeds of Capsicum annuum L by ground simulation from Heilongjiang Academy of Agricultural Sciences as materials, using RAPD （Random Amplified Polymorphic DNA） to detect polyrnorphism of pepper, the influence of different doze of physical simulation on pepper were studied. The results showed that the genetic site could be changed after Li treatment, however, different doze had different effects; many characteristics could be changed by physical simulation, accordingly,this method could produce a complex zenetic variation.

关 键 词：辣椒 地面模拟 RAPD 

分 类 号：S641.3[农业科学—蔬菜学]