番茄青枯病菌拮抗放线菌的筛选及鉴定  

Screening and Identifying of Antagonistic Actinomycetes Against Ralstonia solancearum

作　　者：熊仕俊 孙成龙[3,4] 施闯 蒋选利[1,2] 彭丽娟[1,2] 

机构地区：[1]贵州大学贵州山地农业病虫害重点实验室,贵州贵阳550025 [2]贵州大学农学院,贵州贵阳550025 [3]贵州省农业生物技术重点实验室,贵州贵阳550006 [4]贵州大学生命科学学院,贵州贵阳550025

出　　处：《北方园艺》2014年第5期114-117,共4页Northern Horticulture

基　　金：贵州省科技厅农业攻关资助项目(黔科合NY字[2012]3012);贵州省农业厅资助项目(黔农发[2011]181号;黔财农[2011]136号)

摘　　要：从贵州省贵阳市、遵义市、关岭县、独山县、麻江县等5个市(县),采集20份土壤样品,分离出72株放线菌,采用平板对峙培养法筛选出3株对番茄青枯病菌拮抗效果较好的放线菌,结合形态学特征与16SrDNA序列分析对拮抗放线菌进行鉴定。结果表明:3株拮抗放线菌分别为鲜黄链霉菌(Streptomyces galbus)、栗褐链霉菌(Streptomyces badius)、酸疮痂链霉菌(Streptomyces acidiscabies)。其中,鲜黄链霉菌对青枯雷尔氏菌的拮抗效果最好,其平均抑菌圈直径达55.7mm;栗褐链霉菌的拮抗效果次之,其平均抑菌圈直径为43.2mm;酸疮痂链霉菌平均抑菌圈直径为33.6mm。Seventy-two strains of actinoraycete were isolated from twenty soil samples, which collected from Guiyang, Zunyi, Guanling, Dushan and Majiang in Guizhou province. By pairing culture on plate method, three strains of antagonistic actinomycetes were attained to against Ralstonia solancearum. The results showed that the morphological observation and molecular biology methods identification revealed that the three strains of actinomycetes were Streptomyces galbus , Streptomyces badius and Streptomyces acidiscabies ,among them,the best was S. galbus,and average inhibition diameter was 55. 7 mm. Average inhibition diameter of S. badius was 43. 2 mm,and average inhibition diameter of S. acidiscabies was 33. 6 mm.

关 键 词：番茄 青枯病 生物防治 放线菌 

分 类 号：S641.2[农业科学—蔬菜学]