星点设计效应面法优选穿琥宁固体脂质微粒处方  

Optimization of the Prescription of Potassium Dehydroandrographolide Succinate Solid Lipid Microparticle by Central Composite Design-response Surface Methodology

作　　者：张志勇[1] 王鹏[2] 何文生 

机构地区：[1]四川大学华西医院药剂科,成都610041 [2]核工业四一六医院,成都610051 [3]重庆市大足区人民医院药剂科,重庆402360

出　　处：《中国药房》2014年第11期1011-1013,共3页China Pharmacy

摘　　要：目的:应用星点设计效应面法优选穿琥宁固体脂质微粒的处方。方法:以乳化-溶剂挥发法制备穿琥宁固体脂质微粒,以包封率、平均粒径为评价指标,考察内水相与油相体积比(W1/O)、脂质比(SA/SPC,m/m)、外水相表面活性剂(即泊洛沙姆)质量分数对处方的影响,对结果分别进行多元线性与二项式方程拟合,采用效应面预测最佳处方。结果:最佳处方为W1/O为0.2∶0.2,SA/SPC为8∶1(m/m),泊洛沙姆质量分数为1.0%。二项式拟合方程优于多元线性回归方程,但相关性较差。此条件下,包封率为54.90%、平均粒径为(6.27±0.08)μm。结论:所选处方制备的穿琥宁固体脂质微粒质量合格、重现性好,可为该制剂的进一步研发提供参考。OBJECTIVE： To optimize the prescription of Potassium dehydroandrographolide succinate （PDS） solid lipid mic- roparticle by central composite design-response surface methodology. METHODS： Solid lipid microparticles were prepared by emul- sification-evaporation method. The entrapment efficient and mean particle size were chosen as evaluation indexes to investigate the influence of Wa/O, SA/SPC（m/m）, concentration of external water phase surfactant （poloxamer） on the prescriptions. Multiple lin- ear regression and second-order quadratic models were carried out, and then the optimum prescription was calculated by response surface methodology. RESULTS ： The optimum prescription was as follows： W1/O was 0.2 ： 0.2 ; SA/SPC was 8 ： 1 （m/m）and polox- amer was 1.0%, respectively. Regression coefficients of second quadratic model were higher than multiple linear regression, but there was poor correlation. However, according to the optimal conditions, the entrapment efficient and microparticle size were 54.90% and （6.27 ±0.08）μm. CONCLUSIONS： Prepared PDS solid lipid microparticles is qualified and reproducible, and can provide reference for further R＆D of the preparation.

关 键 词：穿琥宁 固体脂质微粒 星点设计 效应面法 

分 类 号：R284.2[医药卫生—中药学] R28[医药卫生—中医学]