HPLC一测多评法用于木香及3种含木香中药复方制剂的质量评价  

Quality Evaluation of Aucklandia lappa and 3 TCM Compound Preparations Containing A.lappa by HPLC Multi-components Quantitation by One Marker

作　　者：杨宪[1] 刘筱琴 张雪[3] 杨水平[4] 余晓东[1] 和七一[1] 赵瑛[1] 

机构地区：[1]重庆师范大学生命科学学院,重庆401331 [2]重庆化工职业学院应用化学系,重庆400020 [3]重庆市中药研究院,重庆400065 [4]西南大学资源环境学院,重庆400716

出　　处：《中国药房》2014年第11期1030-1034,共5页China Pharmacy

基　　金：重庆市教委科学技术研究项目(No.KJ130618);重庆师范大学校级基金项目(No.12XLB028)

摘　　要：目的：建立测定木香及3种含木香的中药复方制剂中木香烃内酯和去氢木香内酯含量的方法。方法：采用高效液相色谱（HPLc）一测多评法，选取木香烃内酯作为内参物，建立该成分与去氢木香内酯的相对校正因子（f），从而对木香及3种含木香的中药复方制剂中的木香烃内酯和去氢木香内酯进行含量测定。结果：木香烃内酯和去氢木香内酯的进样量分别在0．1012～3．0360gg（r=0．9998）和0．1018～3．0840gg（r=0．9999）范围内与各自峰面积积分值呈良好的线性关系；f=1．0777，用木香烃内酯加佃4得去氢木香内酯的加样回收率为99．53％，RSD=1．11％（n=9）。结论：该试验首次采用HPLC一测多评法测定了木香和3种含木香的中药复方制剂中木香烃内酯和去氢木香内酯的含量，方法准确、可行。OBJECTIVE： To established a method for the content determination of costunolide and dehydrocostus lactone in Aucklandia lappa and 3 TCM compound preparations containing A. lappa. METHODS ： The contents of costunolide and dehydrocos- ms lactone in A. lappa and 3 TCM compound preparations containing A. lappa were determined by HPLC multi-components quanti- tation by one marker, using costunolide as internal standard and the correction factor （f） of costunolide to dehydrocostus lactone. RESULTS： The linear ranges of costunolide and dehydrocostus lactone were 0.101 2-3.036 0 pg（r=0.999 8） and 0.205 6-6.168 0 pg（r=0.999 9）; the f of costunolide was 1.077 7, and average recovery of dehydrocostus lactone was 99.53% （RSD=1.1 1%,n=9）, respectively. CONCLUSIONS： The quantitative method using one marker for simultaneous assay is feasible and applied for quality control ofA. lappa and 3 TCM compound preparations containing A. lappa.

关 键 词：一测多评法 校正因子 木香烃内酯 去氢木香内酯 木香 中药复方制剂 含量测定 

分 类 号：R284.1[医药卫生—中药学] R283[医药卫生—中医学]