消肿接骨贴中苷类成分的定性定量研究  

Qualitative and Quantitative Study of Glycosides in Xiaozhong Jiegu Plasters

作　　者：张金红[1] 房德敏[1] 陈然[2] 高颖[1] 王巨存[1] 郑榕[1] 冯鑫[1] 赵薇[1] 虎冲[2] 

机构地区：[1]天津市天津医院,天津300211 [2]天津医科大学临床医学院,天津300270

出　　处：《中国药房》2014年第11期1037-1039,共3页China Pharmacy

摘　　要：目的：建立消肿接骨贴中苷类成分的定性定量方法。方法：应用薄层色谱（TLC）法对制剂中的续断和黄芪同时进行定性鉴别，以三氯甲烷．甲醇．水（13：7：2，V／V／功的下层溶液为展开剂，10％（V／V）的硫酸乙醇溶液为显色剂。应用高效液相色谱（HPLC）法对制剂中的柚皮苷进行含量测定：色谱柱为ThermoC18（250mm×4．6mm，5μm），流动相为乙腈-水（18：82，刃功，检测波长为283nm。结果：续断和黄芪的TLC斑点清晰、分离良好，阴性对照无干扰。柚皮苷进样量在0．0621-0．6210μg范围内与其峰面积积分值呈良好线性关系（r=0．9997）；精密度、稳定性、重复性试验的RSD〈2％；平均加样回收率为99．3l％，RSD=1．73％（n=9）。结论：本研究建立的方法专属性强、灵敏度高、简便、准确、重复性好，可以用于消肿接骨贴中苷类成分的定性定量研究。OBJECTIVE： To establish a method for qualitative and quantitative study of glycosides in Xiaozhong jiegu plaster. METHODS ：Dipsacus asper and Astragali Radix were identified simultaneously by TLC using chloroform-methyl alcohol-water（ 13： 7：2, V/V/V）as developing solvent and 10 % （V/V）sulfuric acid ethanol as chromogenic agent. HPLC method was used to determine the content of naringin. The determination was performed on Thermo C18（250 min×4.6 mm, 5 μm） with mobile phase consisted of acetonitrile-water（18 ： 82, V/V） and the detection wavelength was set at 283 nm. RESULTS： TLC spots ofD. asper and Astragali Radix were clear and well-separated without interference from negative control. The linear range of naringin was 0.062 1-0.621 0 μg （r=0.999 7） with an average recovery of 99.31% （RSD= 1.73 %, n=9）. RSD of precision, stability and reproducibility tests were all lower than 2%. CONCLUSIONS： The method is specific, sensitive, simple, accurate and reproducible, and it is suitable for qualitative and quantitative identification of glycosides in Xiaozhong jiegu plasters.

关 键 词：消肿接骨贴 苷类 薄层色谱法 高效液相色谱法 

分 类 号：R283.621[医药卫生—中药学] R917[医药卫生—中医学]