将拟南芥ATNCED3基因导入玉米自交系的研究  

Studies on Transferring ATNCED3 into Maize Inbred Line

作　　者：李杰[1,2] 郭欣慰[1,2] 张中保[2] 孙会娟[1,2] 邹华文[3] 罗昌[2] 黄丛林[2] 于荣[1] 吴忠义[2] 

机构地区：[1]首都师范大学生命科学学院,北京100048 [2]北京市农林科学院北京农业生物技术研究中心,北京100097 [3]长江大学农学院,湖北荆州434023

出　　处：《作物杂志》2014年第1期58-62,共5页Crops

基　　金：国家转基因重大专项重点课题(2009ZX08003-009B);北京市科委项目(Z121100001512012和Z111100066111005);北京市农林科学院科研能力创新项目(KJCX201102003)

摘　　要：采用花粉管通道法将ATNCED3基因转入优良玉米自交系昌7-2,在T0代植株三叶期喷洒浓度为200mg/L草铵膦除草剂进行抗性筛选,共得到5株草铵膦抗性植株。对这5株草铵膦抗性植株进行PCR、PCR-Southern及RT-PCR检测,结果表明5株均为阳性植株,初步证明外源目的基因已整合到玉米基因组中,并得到表达。在干旱条件下初步比较了对照和转基因株系叶片的相对含水量、脯氨酸含量,相对电导率等生理生化指标,结果表明,转基因株系各种指标均好于对照,暗示其抗旱性有所提高。The ATNCED3 gene was transformed into maize inbred line ChangT-2 by pollen tube pathway method. The seedlings of three-leaf stage from TO seeds were sprayed with 200mg/L glufosinate screening for herbicide re- sistant lines, and five glufosinate-tolerant plants were obtained. The glufosinate-resistant plants were proved positive by PCR,PCR-Southern and RT-PCR analysis of ATNCED3 gene. The results indicated that ATNCED3 gene was integrated into maize genome and expressed. Physiological indexes such as proline content, relative water content, and relative electric conductivity parameter were measured under drought conditions, and the results showed that transgenic lines were better than control line under water stress ,which indicated that over-expression of ATNCED3 in transgenic lines might increase their drought tolerance.

关 键 词：ATNCED3基因 耐旱性 转基因玉米 花粉管通道法 

分 类 号：S513[农业科学—作物学]