一株植物促生菌的筛选、鉴定及其对花生的促生效应研究  

Isolation, Identification of Plant Growth-promoting Bacteria and Its Promoting Effects on Peanuts

作　　者：徐文思[1] 姜瑛[1] 李引[1] 张振[1] 徐莉[1] 胡锋[1] 李辉信[1] 

机构地区：[1]南京农业大学资源与环境科学学院,南京210095

出　　处：《土壤》2014年第1期119-125,共7页Soils

基　　金：国家自然科学基金项目(30970537)资助

摘　　要：从南京板桥镇自然条件下的潮土中，筛选出JX15菌株，具有较强分泌IAA的能力且性能稳定，20h分泌IAA达到22．55ug／ml，并兼具解磷能力。经形态观察、部分生理生化特征测定及16SrRNA的保守序列鉴定，初步确定该菌株为巨大芽孢杆菌（Bacillus megaterium），GenBank登录号为JX424613。设置单因素试验对菌株生长和发酵条件进行初步研究，结果表明促进菌株JX15生长和增强分泌IAA的最佳培养条件基本一致，最佳培养条件是初始pH7～8，装液量为50ml／250ml，30℃摇床培养24h；最佳碳、氮源分别是甘露醇和酵母粉。在实验室条件下进行花生盆栽试验，结果表明接种JX15菌液的处理，花生植株较未接种菌液的处理其鲜重、株高、全氮、磷、钾及花生根系总长度、根平均直径、根表面积和根尖数均有显著增加。研究结果为该菌株的工程应用提供了理论依据。IAA-producing strains were isolated from fluvo-aquic soil, out of which the strain JX 15 showed the highest and most stable IAA excreting ability （22.55 ug/ml in 20h cultivation）, combining additional phosphate-dissolving capability as well. Based on morphological, part of the physiological and biochemical characteristics and 16S rRNA sequences analysis, it was identified as Bacillus megaterium （GenBank Accession No. JX424613）. Single factor tests were designed for preliminary study of the optimized conditions for the strain growth and fermentation. Results showed that the optimum condition for growth was consistent with that for excretion. The optimal culturing conditions for both growth and IAA secretion were pH 7 - 8, filling volume 50 ml/250 ml flask, incubation at 30℃ for 24 h, the best carbon and nitrogen sources were mannit and yeast extract. These results provided the experimental basis about the strains for the development of bio-organic fertilizer in the future.

关 键 词：潮土 植物促生菌 吲哚乙酸(IAA) 解磷 条件优化 

分 类 号：S154.39[农业科学—土壤学]