重组肺炎克雷伯菌发酵联产3-羟基丙酸和1,3-丙二醇  

Co-production of 3-Hydroxypropionie Acid and 1,3-Propanediol by a Recombinant Strain of Klebsiella pneumoniae

作　　者：李清[1] 黄艳娜[1] 李志敏[1] 叶勤[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《过程工程学报》2014年第1期133-138,共6页The Chinese Journal of Process Engineering

基　　金：国家高技术研究发展计划(863)基金资助项目(编号:2012AA022104)

摘　　要：对表达了高效醛脱氢酶的重组肺炎克雷伯氏菌以甘油为底物生产3．羟基丙酸和1，3-丙二醇的过程进行优化，将发酵过程中补料阶段甘油浓度分别控制为0-10，10-20，20-30g／L，并分3次间歇性补加甘油．结果表明，发酵过程中补料阶段控制甘油浓度在20～30g／L，发酵26h得到47．20g／L3-羟基丙酸和43．90g／L1，3一丙二醇；而间歇性补加甘油产物得率最高，发酵26h时3．羟基丙酸和1,3-丙二醇相对甘油的得率分别为0．35和0．38mol／m01．3-羟基丙酸和1，3-丙二醇联产可实现辅因子烟酰胺腺嘌呤二核苷酸的再生平衡，从而提高碳回收率．The production of 3-hydroxypropionic acid （3-HP） and 1,3-propanediol （1,3-PDO） by recombinant Klebsiella pneumoniae over-expressed aldehyde dehydrogenase was optimized with glycerol as substrate. During the feeding stage of fermentation, glycerol concentration was controlled differently at 0-10, 10-20, 20-30 g/L and intermittent addition of glycerol for three times. The results showed that the process controlling of glycerol concentration at 20-30 g/L resulted in the highest titer, 47.20 g/L of 3-HP and 43.90 g/L of 1,3-PDO were obtained in 26 h. The intermittent addition of glycerol led to the highest yield of 3-HP and 1,3-PDO, 0.35 and 0.38 mol/mol, respectively. The co-production of 3-HP and 1,3-PDO could achieve cofactor regeneration of NAD＋ and NADH balance, which enhanced the recovery of carbon.

关 键 词：肺炎克雷伯氏菌 3 羟基丙酸 1 3 丙二醇 辅因子再生 发酵 优化 

分 类 号：Q819[生物学—生物工程]