鼠胸腺树状突细胞株表达的化学趋化因子的类型分析  

Types of chemokines expressed by murine dendritic cell line

作　　者：郭静竹[1] 龚顺友[2] 谢来平[2] 钱晓萍[2] 陈慰峰[2] 

机构地区：[1]北京大学人民医院儿科,100044 [2]北京大学医学部免疫学系

出　　处：《中华医学杂志》2001年第2期93-96,共4页National Medical Journal of China

摘　　要：目的　分析小鼠胸腺树突状细胞株 (MTDC)中化学趋化因子的类型。方法　以自行设计的引物 ,用逆转录聚合酶链式反应 (RT PCR)方法 ,从小鼠MTDC细胞株扩增SDF 1,IP 10 ,KC等几种化学趋化因子的cDNA片段 ,并对部份扩增产物进行核苷酸测序 ,或dotblotting杂交分析 ,以MTDC浓缩上清进行趋化实验。结果　自MTDC中扩增出了SDF 1、IP 10、KC、MCP 1和Lymphotactin等 5种化学趋化因子的cDNA片段 ,并对SDF 1、KC和Lymphotactin的PCR产物做了核苷酸测序鉴定证明 ,对MCP 1、IP 10和Lymphotactin亦进行dotblotting分析证实。MTDC浓缩上清对小鼠胸腺CD-4 CD-8细胞的趋化指数 (CI)为 3.0 4。结论　MTDC细胞株中存在着多种化学趋化因子。MTDC的浓缩培养上清对小鼠胸腺CD-4 、CD-8细胞具有中等强度的趋化活性 ,符合SDF 1的生物功能。Objective To analyze the types of chemokines expressed in murine medullary thymic dendritic cell line (MTDC). Methods A MTDC line was established. RT PCR was used to amplify the cDNA fragments of SDF 1, IP 10, KC, MCP 1, and lymphotactin expressed by MTDC. Nucleotide sequencing and dot blotting analysis were performed to confirm their existence. Results Five cDNA fragments SDF 1, KC, IP 10, MCP 1, and lymphotactin, were amplified from MTDC. The existence of SDF 1, KC, and lymphotactin was confirmed by nucleotide wequencing, and that of IP 10, MCP 1, and lymphotactin was confirmed by dot blotting. The supernatant of MTDC exhibited chemoattractant activities to CD 4 -～CD 8 - thymocytes with a chemotactic index (CI) of 3.4. Conclusion There is a lot of chemokines in MTDC, including C X C subfamily (SDF 1, KC, and IP 10) CC subfamily (MCP 1), and C subfamily (lymphotactin). The suparnatant of MTDC has moderately chemotactic activities to CD 4 -～CD 8 - thymocytes, consistent with the biological function of SDF 1.

关 键 词：胸腺树突状细胞 趋化因子 化学分析 类型 

分 类 号：R392.1[医药卫生—免疫学]