HPLC同时测定连花清瘟胶囊中绿原酸、连翘苷、大黄酸、大黄素和大黄酚的含量  

Determination of Chlorogenic Acid,Forsythin,Rhein,Emodin and Chrysophanol in Lianhuan Qingwen Capsules by HPLC

作　　者：秦庆芳 

机构地区：[1]广西柳州食品药品检验所,广西柳州545005

出　　处：《中国现代应用药学》2014年第2期210-213,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立HPLC同时测定连花清瘟胶囊中的绿原酸、连翘苷、大黄酸、大黄素和大黄酚的含量。方法采用Agilentc18（4．6mm×250mm，5μm）色谱柱，流动相为乙腈－0．1％磷酸溶液，梯度洗脱（0min→10min→20min→30min→45min±53min，乙腈：10％±10％→20％→30％±68％→75％），流速：1．0mL·min^-1，检测波长：0～15min为327nm，15～35min为205nm，35-43min为230nm，43～48min为221nm，48～55min为224nm；柱温32℃。结果可在55min内完成对绿原酸、连翘苷、大黄酸、大黄素和大黄酚的色谱分析，主成分峰与相邻的色谱峰之间分离度均〉2．0；各测定成分的线性范围分别为0．4445～7．112肛g（，=0．9996），0．1678-2．684μg（r=0．9999），0．031O～0．4963μg（r=0．9995），0．0315～0．504μg（F0．9997），0．0655～1．048μg（r=0．9999）；平均加样回收率（n=9）分别为102．2％（RSD=0．5％），101．0％（RSD=0．8％），98．32％（RSD=0．5％），95．83％（RSD=0．6％1，102．65％（RSD=0.3％）。结论本方法简便，准确，可用于评价莲花清瘟胶囊的质量。OBJECTIVE To develop an HPLC method for determination of chlorogenic acid, forsythin, rhein, emodin and chrysophanol in Lianhuan Qingwen capsules. METHODS The sample was separated on an Agilent C18 column（4.6 mm× 250 mm, 5 μm） with a mobile phase consisting of acetonitrile-0.1% acetic acid in gradient elution（0 min→10min→20min→ 30min→45min→53min, acetonitrile 10%→10%→20%→30%→68%→75%） at the flow rate of 1.0 mL.min^-1 ; at 0-15 rain, the detection wavelength was 327 nm, at 15-35 min, the detection wavelength was 205 nm, at 35-43 min, the detection wavelength was 230 nm, at 43-48 min, the detection wavelength was 221 nm, at 48-55 min, the detection wavelength was 224 nm, and the column temperature was 32 ~C. RESULTS The results showed that all the components were well separated in 55 min by HPLC Each main component chromatographic peak separation resolution was 〉2.0, the linear ranges and correlation coefficients were 0.444 5-7.112 μg（r=0.999 6）, 0.167 8-2.684 μg（r=0.999 9）, 0.031 0-0.496 3 μg（r=0.999 5）, 0.031 5-0.504 μg（r=0.999 7）, 0.065 5-1.048 μg（r=0.999 9）, respectively. The sums of average recovery（n=9） were 102.2%（RSD=0.5%）, 101.0% （RSD=0.8%）, 98.32%（RSD=0.5%）, 95.83%（RSD=0.6%）, 102.65%（RSD=0.3%）. CONCLUSION The method is convenient, accurate, and can be used for quantitative determination of the preparation.

关 键 词：连花清瘟胶囊 高效液相色谱法 绿原酸 连翘苷 大黄酸 大黄素 大黄酚 

分 类 号：R917.101[医药卫生—药物分析学]