小半夏加茯苓汤中鸟苷和腺苷含量的高效液相色谱法测定  

Determination of guanosine and adenosine in Xiaobanxiajiafuling Decoction by HPLC

作　　者：刘育辰[1] 刘刚[1] 何平康[1] 田洪星[1] 何前松[1] 冯泳[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《时珍国医国药》2014年第2期277-279,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81160425/30960472);贵州省科技计划(黔科合中药字【2011】LK7030号)

摘　　要：目的建立测定小半夏加茯苓汤中乌苷和腺苷含量的HPLC分析方法。方法色谱柱为Diamonsil C18柱（4．6mm×250m／F／，5μm），流动相为乙腈－0．01％醋酸水溶液，梯度洗脱，检测波长25411171，体积流量为1．0ml·min^-1，柱温30℃。结果鸟苷和腺苷分别在0．031—1．027μg（r=0．9999）和0．015—1．010μg（r=1．0000）内线性关系良好，平均加样回收率分别为102．8％、102．5％，RSD分别为1．93％、1．8％（n=6）。结论该方法简便、可靠、重现性好，可用于测定小半夏加茯苓汤中鸟苷和腺苷的含量。Objective To establish an HPLC method for the content determination of guanosine and adenosine in Xiaobanxiajia- fuling Decoction. Methods The Diamonsil Cls column was used. The mobile phases consisted of acetonitrile and 0.01% ethanoic acid with gradient elution. The detection wavelength was at 254 rim. The flow rate was 1.0 ml. min^ -1 and column temperature was at 30 ℃. Results Guanosine and adenosine were linear in range of 0. 031 - 1. 027 μg （r = 0. 999 9） and 0. 015 - 1. 010 μg （r = 1. 000 0）respectively. The average recoveries were 102.8% and 102.5% with the RSD 1.93% and 1.8% （n = 6） respectively. Conclusion The method is rapid, accurate, reliable and can be used to determine the guanosine and adenosine in Xiaobanxiajiafuling Decoction.

关 键 词：小半夏加茯苓汤 鸟苷 腺苷 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]