甜瓜自毒作用相关基因CmTIP的克隆和分析  

Cloning and analysis of autotoxicity related gene CmTIPin melon

作　　者：谢新蕊[1] 高强[1] 奚玉培[1] 张志忠[1] 

机构地区：[1]福建农林大学园艺学院,福建福州350002

出　　处：《河北农业大学学报》2014年第1期44-48,63,共6页Journal of Hebei Agricultural University

基　　金：福建农林大学园艺学院青年学术骨干培养基金"甜瓜化感作用机理研究"(FAFU2012YYPY08);福建省科技重大专项"蔬菜优质高效配套栽培技术研究"(2008NZ0002-1)

摘　　要：以甜瓜品种‘新银辉’为试验材料,利用cDNA-AFLP技术分离并获取了甜瓜自毒作用相关水通道蛋白基因片段序列,利用RACE技术获得该基因全长cDNA序列,包含750bp的完整阅读框,编码250个氨基酸,具有6个跨膜区,2个高度保守的水孔蛋白基序NPA以及MIP特征序列,命名为:CmTIP(GenBank登录号:KF134811)。Blastn分析显示该基因与黄瓜CsTIP1;1基因同源性较高;荧光定量PCR分析结果显示该基因在根系分泌物胁迫处理6d时表达量最高,这与cDNA-AFLP分析结果一致。Differential fragments of tonoplast intrinsic protein （TIP） were analyzed by the cD- NA-AFLP technique and obtained from the leaves of melon, which associated with autotoxicity using ＇New Silverlit＇ as the test material, which was cloned by RACE technology. The full-length cDNA sequence of CmTIP had an ORF of 750 bp and encoded 250 amino acids. Six transmembrane domains, two highly conserved NPA motifs of aquaporins and the characteris- tics sequence of the MIP family were found in CrnTIP. Therefore, the gene of melon could be named as CmTIP（GenBank accession number. KF134811）. The results showed that this frag- ment was highly associated with TIP1 ;1 genes of cucumber; the result of fluorescence quanti- tative PCR showed that the gene expressed highest on the 6th day after root exudates treat- ment, which was consistent with the results of cDNA-AFLP.

关 键 词：甜瓜 自毒作用 水通道蛋白 根系分泌物 

分 类 号：S652[农业科学—果树学]