有斑百合花粉低温贮藏研究  

Studies on cryopresevration of Lilium concolor var.pulchellum

作　　者：赵婵璞[1] 张泉卫 史宝胜[1] 刘冬云[1] 

机构地区：[1]河北农业大学园林与旅游学院,河北保定071000 [2]大连软件园安博开发有限公司,辽宁大连116000

出　　处：《河北农业大学学报》2014年第1期54-58,共5页Journal of Hebei Agricultural University

基　　金：河北省教育厅项目:河北省野生耐阴性地被植物资源的收集筛选及应用研究(2010253)

摘　　要：以有斑百合为试材，研究了不同贮藏温度、花粉含水量、冻存方式及解冻方式对有斑百合花粉萌发率的影响。结果表明：低温冷冻保存是实现有斑百合花粉长期保存一个有效的途径，-80℃贮藏可以使花粉保持2年的生命力；有斑百合花粉在室温下干燥脱水处理8～12h，含水量最适宜范围为18％～22％，效果较好；采用液氮预冻再投入中-80℃保存，有效提高了超低温保存后有斑百合花粉的萌发率；采用35℃温水快速化冻和0℃化冻方式，均能保持较好的冷冻低温花粉保存效果。Taking Lilium concolor var. pulchellum as the test material,effects of different stor- age temperature, water content of pollen, pre-freezing and thawing methods on germination rate of pollens were investigated. The results showed that cryopreservation is an effective way to achieve long-term preservation in the pollens of Lilium concolor var. pulchellum, it can make the vitality of pollens maintain for 2 years under -80℃; a moisture range of 18%--22% was suit for cryopreservation of pollens in Lilium concotor var. pulcheltum, and it was better that it took 8-12 h to dry and dehydrate by the dry dish at room temperature; it can effectively improve the germination rate after cryopreservation by the freezing of liquid nitrogen firstly and then preserved in -80℃ ;and it can keep a better preservation effect by the way in 35 ℃ warm rapid thaw or 0℃ thaw.

关 键 词：有斑百合 花粉 萌发率 低温保存 

分 类 号：S682.2[农业科学—观赏园艺]