Box-Behnken设计-效应面法优选防风炮制工艺  

Optimization of Processing Technology for Saposhnikoviae Radix by Box-Behnken Design-Response Surface Methodology

作　　者：刘婵[1] 张水寒[1,2] 黄惠勇[1] 秦裕辉[1] 肖娟[1,2] 蔡萍[1,2] 万丹[1,2] 

机构地区：[1]湖南省中医药研究院,长沙410003 [2]杨永华全国名老中医药专家传承工作室,长沙410003

出　　处：《中国实验方剂学杂志》2014年第5期18-21,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖南省科技厅重点项目(2012TF1005)

摘　　要：目的：优选防风的炮制工艺.方法：以升麻素苷和5-O-甲基维斯阿米醇苷含量、浸膏得率、水分的综合评分为指标,采用Box-Behnken设计-效应面法考察切片厚度、干燥时间、干燥温度对防风饮片质量的影响,优选防风饮片的炮制工艺参数.结果：最佳炮制工艺为切片厚度3 ～4 mm,干燥温度60℃,干燥时间2h.升麻素苷和5-O-甲基维斯阿米醇苷的质量分数分别为0.917％,0.054％,水分5.110％,浸膏得率20.45％.结论：采用Box-Behnken设计-效应面法优选的炮制工艺合理可行,为防风饮片的质量控制提供参考.Objective：To optimize processing technology of Saposhnikoviae Radix.Method：Taking composite score of contents of prim-O-glucosylcimifugin and 5-O-methylvisammioside,extract yield,water contents as index,effects of slice thickness,drying time and drying temperature on pieces quality were investigated by BoxBehnken design-response surface methodology,so as to optimize processing technology parameters of Saposhnikoviae Radix.Result：Optimal processing parameters were as follows：slice thickness 3-4 mm,drying temperature at 60 ℃,drying time 2 hours.Under these conditions,contents of prim-O-glucosylcimifugin and 5-O-methylvisammioside,extract yield,water contents were 0.917％,0.054％,20.45％ and 5.110％,respectively.Conclusion：Optimized processing technology for Saposhnikoviae Radix was feasible and reasonable,it could provide a reference for quality control of Saposhnikoviae Radix pieces.

关 键 词：防风 炮制工艺 BOX-BEHNKEN设计 响应曲面法 升麻素苷 5-O-甲基维斯阿米醇苷 

分 类 号：R283.3[医药卫生—中药学]