中药鬼箭羽质控标准的建立  

Establishment of the Criteria of Quality Control for Chinese Medicinal Herb Euonymus alatus

作　　者：李明江[1] 张玉玲[1] 潘扬[1] 张弦[1] 

机构地区：[1]南京中医药大学药学院,南京210029

出　　处：《中国实验方剂学杂志》2014年第5期60-66,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏高校优势学科建设工程项目(ysxk-2010)

摘　　要：目的:建立中药鬼箭羽形态、组织学的特征体系及其指标性成分的定性和定量方法,为鬼箭羽的药材质量评估提供科学依据。方法:以10批不同来源的鬼箭羽样本作为研究对象,生药学制片技术观察组织构造特征;参照2010年版《中国药典》方法测定水分、浸出物和灰分;以柚皮素为对照品进行硅胶TLC法定性鉴别;分别采用紫外分光光度法和HPLC测定鬼箭羽中总黄酮和柚皮素的含量。结果:10批鬼箭羽药材的显微特征基本一致,不同产地药材并不存在显著的差异。各药材水分为13.8%±0.9%;水浸出物5.9%±0.5%,醇浸出物4.7%±0.5%;总灰分8.4%±1.8%,酸不溶性灰分2.9%±1.0%。供试品TLC色谱中,在与柚皮素对照品色谱相对应的位置上均显相同的红色斑点。总黄酮含量为2.7%±0.7%,柚皮素0.006%±0.005%。结论:实验结果可以作为评判中药鬼箭羽质量好坏的参考标准。Objective：To provide scientific basis for quality evaluation of Chinese medicinal herb Euonymus alatus by studying the identification of morphological and histological characteristics,and setting up qualitative and quantitative methods for analyzing the characterized components of E.alatus.Method：Focusing on ten different sources of E.alatus,the paraffin section and photomicrographic techniques were used to describe microscopic characters.The content of moisture,ash,solvent extract was determined according to Chinese Pharmacopoeia （Ed.2010） methods.The silica gel TLC identification of E.alatus was performed by using naringenin as a reference substance.The content of total flavonoids and naringenin was also determined by UV-spectrophotometry and HPLC respectively.Result：The microscopic characters of ten different sources of E.alatus were basically the same pattern without striking differences.The content of moisture was 13.8％ ±0.9％,aqueous extracts 5.9％ ± 0.5％,alcoholic extracts 4.7％ ± 0.5％,total ash 8.4％ ± 1.8％,and acid-insoluble ash 2.9％ ± 1.0％.The developed TLC spots were quite clear,and a red spot obtained with the given samples all corresponded in position and color to the spot obtained with the reference naringenin in the chromatogram.The content of total flavonoids was 2.7％ ± 0.7％,and naringenin 0.006％ ± 0.005％.Conclusion：All these data mentioned above can be used as the reference standards for evaluating quality of E.alatus.

关 键 词：鬼箭羽 质量控制 总黄酮 柚皮素 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]