胶束电动毛细管电泳法快速分离测定南、北五味子中木脂素类成分  

Rapid Determination of Six Lignans in Schisandra Chinensis by Micellar Electrokinetic Capillary Chromatography

作　　者：李坦[1] 孙绩岩 苑广信[2] 孙新[1] 

机构地区：[1]北华大学生命科学中心,吉林132013 [2]北华大学药学院,吉林130021

出　　处：《中国实验方剂学杂志》2014年第5期67-71,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省科技发展计划项目(20111809)

摘　　要：目的：建立胶束电动毛细管电泳法测定五味子中6种木脂素类成分的方法.方法：探讨了缓冲溶液、添加剂、分离电压、温度和进样条件等因素对分离检测的影响.在10 mmol·L-1NaH2PO4-Na2HPO4缓冲溶液（35％乙腈,37.5 mmol·L-1SDS,pH 8.0）、分离电压为28.0 kV的优化条件下,9 min内可实现五味子样品溶液的分析.结果：线性范围分别为25.5 ～1020,25.75～1 030,12.0 ～480,25.5～1 020,24.25～970,19.75 ～ 790 mg· L-1;检出限为0.5,0.8,1.0,1.0,1.0,1.5 mg·L-1.结论：该方法与高效液相色谱法比较,在分离效果、柱效和分析速度方面具有明显优势.Objective：A micellar electrokinetic capillary chromatography （MEKC） was developed for the rapid determination of six lignans in Schisandra chinensis.Method：The key factors for separation and determination were studied and the best analysis conditions were obtained,in the background electrolyte of 10 mmol · L-1 phosphate （35％ acetonitrile,37.5 mmol ·L-1 SDS,pH 8.0） at the separation voltage of 28.0 kV,the Schisandra extract could be detected within 6 min.Result：The lignans showed good linear response in the range of 25.5-1 020,25.75-1 030,12.0-480,25.5-1 020,24.25-970 and 19.75-790 mg ·L-1,the limit of detection was0.5,0.8,1.0,1.0,1.0 and 1.5 mg ·L-1.Conclusion：Compared with high-performance liquid chromatography （HPLC）,the MEKC analyses offers high separation efficiencies and rapid analyses.

关 键 词：胶束电动毛细管电泳法 五味子 木脂素 

分 类 号：R284.1[医药卫生—中药学]