6味山姜属中药对胃实寒证大鼠胃组织PDE,cAMP,cGMP,cAMP/cGMP的影响  

Effect of Six Alpinia Roxb on PDE,cAMP,cGMP,cAMP /cGMP of Gastric Tissue of Stomach in Excessive Cold Syndrome Rats

作　　者：黄燕琼[1] 秦华珍[2] 柳俊辉[2] 王晓倩[2] 刘磊[2] 余腾飞[2] 刘颖[2] 李文强[2] 谭喜梅 

机构地区：[1]广西中医药大学附属瑞康医院,南宁530011 [2]广西中医药大学,南宁530001

出　　处：《中国实验方剂学杂志》2014年第5期161-165,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西教育厅课题(201012MS144);广西中医学院课题(P2009043)

摘　　要：目的：研究高良姜、草豆蔻、红豆蔻、大高良姜、建砂仁、益智6味山姜属中药对胃实寒证模型大鼠胃组织磷酸二酯酶（PDE）、环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）、cAMP/cGMP的影响,揭示6味山姜属中药所含挥发油与温中散寒功效的相关性.方法：实验分2批进行,第1批取SD雄性大鼠160只分为16个组：模型Ⅰ组,模型Ⅱ组,阳性对照组,高良姜、草豆蔻、红豆蔻3味药物各个挥发油高、低剂量组、去挥发油水液高、低剂量组;第2批除了观察药为大高良姜、建砂仁、益智挥发油高、低剂量组、去挥发油水液高、低剂量组外,其余分组均同第1批.采用灌服2～3℃寒凉药（石膏、知母、黄柏、龙胆草,给药剂量为36g·kg-1,2次/d）加冷冻的方法制造大鼠胃实寒证模型;分别予以6味药物挥发油、去挥发油水液,低剂量组给药剂量为20g·kg-,高剂量组给药剂量为60 g·kg-1,阳性对照药选用附子水煎液,剂量为8 g·kg-1;每隔12 h给药1次,连续给药3次.测定大鼠胃组织PDE,cAMP,cGMP含量,计算cAMP/cGMP.结果：①对磷酸二酯酶（PDE）的影响：与正常组相比,第1,2批模型组胃组织中PDE明显升高（P＜0.05或P＜0.01）,分别为32.27,52.80 nmol·L-1.高良姜、草豆蔻、红豆蔻、大高良姜、益智的挥发油高、低剂量组对于模型组大鼠胃中PDE有显著的降低作用（P＜0.05或P＜0.01）,大高良姜去挥发油高剂量组对其也有一定的降低作用,但无显著性差异;②对cAMP,cGMP及cAMP/cGMP的影响：与空白组比较,第1,2批模型组胃组织中cAMP的浓度明显降低（P＜0.05）,分别为0.68,0.81 nmol·L-1.6味中药挥发油高、低剂量组与模型组相比,cAMP浓度均明显升高（P ＜0.05或P＜0.01）,cGMP无明显变化,cAMP/cGMP增大（P＜0.05或P＜0.01）;高良姜、草豆蔻、建砂仁去挥发油高剂量组对cAMP浓度也有一定的影响,但无显著性差异.结论：6味中药所含挥发油可升高�Objective：To study the effect of the six Alpinia Roxbe on phosphodiesterase （PDE）,cyclic Adenosine monophosphate （cAMP）,cyclic guanosine monophosphate （cGMP）,cAMP/cGMP of the gastric tissue of stomachin excessive cold syndrome rats,and reveal the correlation between the six kinds volatile oil of Alpinia Roxb.and the function of warming middle energizer and dispersing cold.Method：By giving cold-cool nature Chinese medicines and freezing method,stomach excessive cold syndrome rat models was established.The high and low dose of the volatile oil and the water extract removed volatile oil of the six Chinese medicine were given to rat,the positive control drug was given water extract of aconite.PDE,cAMP and cGMP,and calculated cAMP/cGMP ratio were determined.Result：①The high and low dose groups of the volatile oil of the six Chinese medicine significantly reduced the concentration of stomach PDE in model rats （P ＜ 0.05 or P ＜ 0.01）,high dose group of water extract removed volatile oil of Alpinia galanga also had a certain reduction effect,but no significant difference.②The high and low dose groups of the volatile oil of the six Chinese medicine compared with model group,cAMP concentrations were significantly increased （P ＜ 0.05 or P ＜ 0.01）,cGMP no significant changes,cAMP/cGMP ratio increased （P ＜0.05 or P ＜0.01） ; the high dose group of water extract removed volatile oil of galangal,Alpinia katsumadai,Villous amomum fruit also had some influence on the concentration of cAMP,but no significant difference.Conclusion：The volatile oil of the six Alpinia Roxb may elevate gastric tissue cAMP levels,cAMP/cGMP ratio,reduce the PDE content,some Chinese medicine without volatile oil at high dose also had a similar effect.The volatile oil of the six Alpinia Roxb was related the function of warming middle energizer and dispersing cold,and water extract removed volatile oil of the six Chinese medicine also had some good improvement,revealing their effectiveness is also associated wi

关 键 词：山姜属 挥发油 去挥发油水液 胃实寒证 磷酸二酯酶 环磷酸腺苷 环磷酸鸟苷 

分 类 号：R285.5[医药卫生—中药学]