复方玉郎伞多糖对鸭乙型肝炎病毒诱导的肝损伤的影响  

Effect of Compound Yulangsan Polysaccharide against Duck Liver Injury Induced by Duck Hepatitis B Virus

作　　者：陶丽群 左巧云 李秀菊[1] 陈春霞[1] 黄仁彬[1] 

机构地区：[1]广西医科大学药理教研室,南宁530021

出　　处：《中国实验方剂学杂志》2014年第5期172-176,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科学研究与科技开发攻关项目(桂科攻10124008-6;桂科攻0992003A-2);广西科技基础条件平台建设项目(12-97-20;10-108-19);广西地方性高发疾病防治研究重点实验室基金(KFJJ2010-22)

摘　　要：目的:研究复方玉郎伞多糖(CYLSP)在鸭乙型肝炎病毒(DHBV)持续性感染模型中对DHBV所致的肝损伤的保护作用。方法:采用1 d龄雏鸭接种广西麻鸭乙型肝炎病毒(DHBV)强阳性血清,接种1周后用聚合酶链式反应(PCR)法筛选出DHBV强阳性鸭50只,随机分为5组,每组10只,CYLSP高、中、低剂量组(10,5,2.5 g·kg-1)、拉米夫定(0.05 g·kg-1)组和模型组,每日上午ig给药1次,连续14 d。于用药前(T0)、用药7 d(T7)和14 d(T14)及停药后3 d(P3)分别采血,同时检测血清中丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的活性;在停药3 d后,取肝脏匀浆并除蛋白,检测肝匀浆液中总超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性以及谷胱甘肽(GSH)、丙二醛(MDA)的含量;HE染色处理肝脏组织切片,光镜观察病理学改变。结果:与同组T0及模型组比较,各给药组鸭血清高、中剂量治疗组给药7 d(T7)和14 d(T14)即能明显降低血清ALT,AST的活性,停药3(P3)天后,CYLSP高剂量组仍能显示出持续有效,没有出现反跳现象(P<0.05或P<0.01);检测肝匀浆液中SOD,GSH-Px活性以及GSH,MDA的含量,CYLSP高、中剂量组和拉米夫定组仍能显示出持续有效,没有出现反跳现象(P<0.05或P<0.01);HE染色显示CYLSP可显著减轻鸭肝组织病理损伤程度。结论:CYLSP对DHBV所致的肝损伤具有保护作用。Objective：To study the inhibitory effect of compound Yulangsan polysaccharide （CYLSP） on duck hepatitis B virus （DHBV） persistent infection.Method：One-day old Guangxi brown spotted ducks infected with hepatitis B virus （DHBV） were used as the DHBV infected animal model.Fifty positive ducks were detected by PCR at one week after the infection of DHBV,then randomly divided into 5 groups,each group of 10,only CYLSP high,medium and low dose group （10,5,2.5 g ·kg-1） and lamivudine （0.05 g·kg-1） group and model group,the daily morning lavage l time for 14 d.Alanine aminotransferase （ALT）,aspartate aminotransferase （AST） in serum were detected before treatment （T0）,7 days （T7）,14 days （T14） after treatment,and 3 days after stopping treatment.At 3 days after stopping treatment,the livers were collected,homogenate was prepared to detect superoxide dismutase （SOD）,glutathione peroxidase （GSH-Px） activity and reduced glutathion （GSH）,malondialdehyde （MDA） levels,HE staining was used to observe the pathological change.Result：Compared with T0 and model group,the serum levels of ALT and AST were decreased significantly and 3 days after stopping treatment,CYLSP high dose group and lamivudine group still showed a protection effect （P ＜ 0.05 or P ＜ 0.05） SOD,GSH-Px activity and the content of GSH,MDA.CYLSP in high,middle dose group and lamivudine group showed a protection action.In CYLSP low dose group,no change of SOD,GSH-Px activity and GSH,MDA content was found,HE staining showed CYLSP significantly reduced the duck liver histopathologic damage degree.Conclusion：The compound Yulangsan polysaccharide has a protection action on duck liver injury induced by duck hepatitis B virus

关 键 词：复方玉郎伞多糖 鸭乙型肝炎病毒 丙氨酸转氨酶 天冬氨酸转氨酶 超氧化物歧化酶 谷胱甘肽过氧化物酶 谷胱甘肽 丙二醛 

分 类 号：R285.5[医药卫生—中药学]