急性主动脉夹层与正常对照主动脉组织基因的差异表达  

Analysis of the Differentially Expressed Genes in Aorta Tissue in Patients with Acute Aortic Dissection

作　　者：王晓建[1] 黄毕[2] 张良[3] 苏文君[3] 路天怡[3] 田力[2] 杨艳敏[2] 张澍[2] 樊晓寒[2] 惠汝太 

机构地区：[1]中国医学科学院北京协和医学院国家心血管病中心阜外心血管病医院心血管疾病国家重点实验室,北京市100037 [2]中国医学科学院北京协和医学院国家心血管病中心阜外心血管病医院心内科,北京市100037 [3]中国医学科学院北京协和医学院国家心血管病中心阜外心血管病医院心外科,北京市100037

出　　处：《中国分子心脏病学杂志》2014年第1期812-815,共4页Molecular Cardiology of China

基　　金：国家自然科学基金(81170286)

摘　　要：目的通过组织芯片技术分析急性主动脉夹层患者主动脉组织差异表达基因探讨主动脉夹层发病的分子机制。方法取急性主动脉夹层患者及健康对照(各4例)的主动脉组织,通过组织芯片筛选急性主动脉夹层患者和健康对照差异表达的基因。利用生物信息学技术分析差异表达的基因参与的信号通路。结果实主动脉组织芯片分析结果显示,急性主动脉夹层患者与正常对照差异表达大于2倍的基因有129个,其中83个基因表达下调,46个基因表达上调。差异表达的基因主要编码与细胞、细胞外基质粘附相关的蛋白。信号通路分析显示,主动脉夹层中受影响最大的两条通路是粘附斑和肌动蛋白骨架调节相关通路。结论通过组织芯片分析筛选到的急性主动脉夹层差异表达基因可能参与了主动脉夹层的病理过程,为研究其致病机制奠定了基础。Objective To gain a better understanding of the molecular mechanisms underlying acute aortic dissection （AAD） by analyzing the differentially expressed genes in patients with AAD. Methods Aortic tissue was collected from AAD patients （n=4） and control （n=4）. The AffymetrixGeneChip Human Genome U 133 Plus 2.0 array was utilized. Bioinformatics sofiwares were used to analyze the biologic function and pathways of the differentially expressed genes. Results We found 129 differentially expressed genes with more than 2- fold changes by gene microarray, of which 83 were down-regulated and 46 were up-regulated in patients with AAD compared with normal controls. These dysregulated genes mainly code proteins related with cell-cell and cell-matrix adhesion by bioinformatics analysis and the most affected pathways in AAD are focal adhesion and actin cytoskeleton regulation. Conclusion The differentially expressed genes detected by microarray in patients with AAD compared with controls may contribute to the pathogenesis of AAD.

关 键 词：组织芯片 急性主动脉夹层 基因差异表达 

分 类 号：R543.1[医药卫生—心血管疾病]