人面果叶子、根部、果实提取物体外抗糖尿病活性研究(英文)  

In Vitro Assessment of Antidiabetic Activities of Garcinia Xanthochymus Leaf,Root and Fruit Extracts

作　　者：付蒙[1,2] 胡鑫[1] 徐婧[1] 陈玉[3] 杨光忠[1] 

机构地区：[1]中南民族大学药学院,武汉430074 [2]湖北省食品药品监督检验研究院,武汉430064 [3]中南民族大学化学与材料科学学院,武汉430074

出　　处：《天然产物研究与开发》2014年第2期255-259,共5页Natural Product Research and Development

基　　金：State Ethnic Affairs Commission Research Project;National Natural Science Foundation of China(No.31370379)

摘　　要：为了评价人面果叶子、根部、果实提取物体外抗糖尿病活性，相应测定了其石油醚提取物（PFr．）、乙酸乙酯提取物（EFr．）、正丁醇提取物（BFr．）、水提取物（WFr．）的α-葡萄糖苷酶与仪-淀粉酶抑制活性，以及HepG2细胞的促葡萄糖消耗能力。果实乙酸乙酯提取物（IC50=17．81±1．09μg/mL）、叶子乙酸乙酯提取物（IC50=18．60±1．56μg／mL）、根部乙酸乙酯提取物（IC50=14．05±0．24μg／mL）、根部正丁醇提取物（IC50=13．01±0．38μg／mL）显示了较好的仪一葡萄糖苷酶抑制活性（aearb086IC50〉200．μg/mL）。而根部乙酸乙酯与正丁醇提取物在600μg／mL的浓度下就显示了90％的α-葡萄糖苷酶抑制率，在1．5mg／mL的浓度下显示了90％的α-淀粉酶抑制率。在促葡萄糖消耗试验中，果实乙酸乙酯提取物在浓度为7．5～30mg／mL时显示了很好的促HepG2细胞葡萄糖消耗能力（P〈0．001），叶子乙酸乙酯提取物、根部正丁醇与乙酸乙酯提取物的促葡萄糖消耗率达到了3．08、3．12、1．93，仅次于果实乙酸乙酯提取物（3．91）。此次研究为人面果抗糖尿病活性开发提供一定理论基础。The objective of this study was to evaluate the antidiabetic potential of Garcinia Xanthochymus in vitro. The α- glucosidase and α-amylase inhibitory activities of the petroleum ether （ PFr. ）, ethyl acetate （ EFt. ）, n-butanol （ BFr. ） and water fractions（WFr. ） of the tcot,leaf and fruit of G. Xanthochymus were assessed. Glucose consumption was evalu- ated using HepG2 cells. Fruit-EFr（IC50 = 17.81 ± 1.09 μg/mL）, leaf-EFt（ ICso = 18.60± 1.56 μg/mL）, rcot-EFr （ IC50 = 14.05 ± 0.24μg/mL） and rcot-BFr（ IC50 = 13.01 ± 0.38 μg/mL） showed higher α-glueosidase inhibitory activity than aearbose（IC50 〉 200 μg/mL）. The root EFr and BFr showed the highest α-glucosidase inhibitory activity among all samples（ca. 90% ,at a concentration of 600 μg/mL）. The a-amylase inhibitory activity rate of the root EFr and BFr reached 90% at a concentration of 1.5 mg/mL. In glucose consumption assay,four fractions were efficient on promoting glucose consumption activity. The fruit-EFr at a concentration of 7.5-30 mg/mL signitlcanfly promoted glucose consumption（P 〈 0.001 ） and the glucose consumption rates of cells treated with leaf-EFr,root-BFr and reot-EFr were 3.08,3.12 and 1.93 ,respectively,slightly lower than the glucose consumption rate of cells treated with fruit-EFt. These findings supported the antidiabetic claims of G. xanthochymus in vitro.

关 键 词：人面果 抗糖尿病 仪-葡萄糖苷酶抑制活性 α-淀粉酶抑制活性 HepG2细胞葡萄糖消耗试验 

分 类 号：R932[医药卫生—生药学]