白藜芦醇预处理对束缚应激大鼠胃溃疡的保护作用  

Protective Effects of Resveratrol Preconditioning on Stress Gastric Ulceration of Rats

作　　者：张红[1] 问慧娟[2] 周雅静[2] 姜源涛[1] 

机构地区：[1]河北大学附属医院 [2]河北大学基础医学院,保定071000

出　　处：《天然产物研究与开发》2014年第2期260-262,299,共4页Natural Product Research and Development

基　　金：河北省中医药类科研计划项目(2012134);河北省科学技术研究与发展计划项目(11276103D-7);河北省卫生厅重点科技研究计划项目(20100141)

摘　　要：采用水浸束缚应激(WRS)诱导大鼠急性胃粘膜损伤模型,观察胃粘膜氧化应激指标和胃液pH值的变化,探讨白藜芦醇对大鼠应激性胃溃疡的保护作用及机制。将30只wistar大鼠随机分为对照组,应激组,白藜芦醇低、中、高剂量组(30、60、120 mg/kg)。通过观察胃黏膜形态学改变,测定胃液pH值、胃溃疡指数(UI)、胃黏膜组织SOD活性及MDA含量的改变,研究不同剂量白藜芦醇对大鼠应激性胃溃疡的抑制作用及机制。结果显示白藜芦醇能明显减轻WRS大鼠胃黏膜的水肿、出血和溃疡面积,明显降低UI(P<0.01);同时显著提高胃粘膜组织SOD的活性、胃液pH(P<0.01)和降低MDA水平(P<0.01),各指标均以白藜芦醇中剂量组作用显著。本实验结果表明白藜芦醇具有较强的抗溃疡作用,对束缚应激大鼠胃溃疡有明显保护作用,其机制主要与减少自由基产生和提高抗氧活酶活性有关。In this study, the protective effect of resveratrol on gastric mucosal damage induced by water-immersion re- straint stress（WRS） in rats was investigated. 30 wistar rats were randomly divided into five experimental groups ：control group, model group,low dose resveratrel（Res） group （30 mg/kg）, middle dose Res group （60 mg/kg） and high dose Res group（ 120 mg./kg）. Macroscopic gastric lesions were evaluated, the ulceration index and pH of gastric juice was measured, the content of malondialdehyde （MDA） and the activity of super oxide dismutase （SOD） in gastric mucosa were measured. The results showed that resveratrol significantly reduced the ulcer area induced by WRS and inhibited the Ulcer Index. Middle dose Res group showed the best result（ P 〈 0.01 ） ; It significantly improved the SOD（P 〈 0.01 ） and pH of gastric juice,while reduced content of MDA（P 〈0.01 ）. The present study suggested that resveratrol was able to protect the gastric mucosa which may be mediated by improving the antioxidant live enzyme activity and restricting free radical production during stress.

关 键 词：白藜芦醇 应激 溃疡指数 SOD MDA 

分 类 号：R285.5[医药卫生—中药学]