药用植物内生菌对青藤碱的微生物转化筛选  

Screening of Endophytic Fungi from Medicinal Plant for Biotransformation of Sinomenine

作　　者：刘发贵[1] 潘烨[1] 邓艾平[1] 罗丹[1] 杨进[1] 郭志勇[1] 邓张双[1] 

机构地区：[1]三峡大学天然产物研究与利用湖北省重点实验室,宜昌443002

出　　处：《天然产物研究与开发》2014年第2期268-272,共5页Natural Product Research and Development

基　　金：国家自然科学基金项目(31100080);湖北省自然科学基金项目(2011CDB177);三峡大学人才科研启动基金项目(KJ2011B003)

摘　　要：为了获得具有抗炎活性的青藤碱衍生物,建立了药用植物内生菌对青藤碱的生物转化模型,从84株药用植物内生菌中筛选出5株对青藤碱具有转化能力的菌株,利用TLC和NMR技术对转化产物进行了结构鉴定,确认为(S)-disinomenine,(R)-disinomenine和sinomenine N-oxide。生物转化为青藤碱的结构改造提供了研究方向。In order to obtain sinomenine derivatives with anti-inflammatory activity, biotransformation of sinomenine was performed using endophytic fungi from medicinal plants. Among 84 endophytic fungi isolated from medicinal plants,five fungi possessed the capability of biotransforming sinomenine were screened out. The structures of metabolites were identi- fied as （S）-disinomenine, （R）-disinomenine and sinomenine N-oxide by TLC and NMR. Biotransformation is going to provide a new research direction for structural modification of sinomenine.

关 键 词：药用植物 内生菌 微生物转化 青藤碱 

分 类 号：Q819[生物学—生物工程]