体外添加不同水平的亚麻籽油对气体产量、瘤胃发酵及脂肪酸组分的影响  

Effects of Addition of Different Levels of Linseed Oil on Gas Production, Rumen Fermentation and Proportion of Fatty Acids in vitro

作　　者：吴端钦[1,2] 贺志雄[1] 汤少勋[1] 谭支良[1] 

机构地区：[1]中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,湖南省畜禽健康养殖工程技术中心,农业部中南动物营养与饲料科学观测实验站,长沙410125 [2]中国科学院大学,北京100049

出　　处：《天然产物研究与开发》2014年第2期273-277,共5页Natural Product Research and Development

基　　金：中国科学院战略性先导科技专项(XDA05020700)

摘　　要：利用瘤胃体外产气法研究在羊草底物条件下添加不同水平的亚麻籽油(LSO)对气体产量、瘤胃发酵和脂肪酸组分的影响。LSO添加水平分别为底物干物质的5%和10%,体外培养持续48 h。在12、24和48 h时测量总气体产量、甲烷及氢气产量,培养结束后测定发酵指标和发酵液中脂肪酸成分。结果表明,LSO显著降低了产气量和甲烷产量,提高了氢气产量;添加LSO显著提高了总挥发性脂肪酸含量、丙酸和丁酸的比例,还显著降低了发酵液pH和氨氮浓度;同时,添加LSO提高了发酵液中对人体健康有益的共轭亚油酸以及其他不饱和脂肪酸的比例。这些结果表明,富含十八碳不饱和脂肪酸的LSO可以在粗饲料条件下抑制甲烷生成,改善瘤胃发酵,增加了多不饱和脂肪酸的含量,而且抑制甲烷生成及改善瘤胃发酵的效果与添加剂量有关。The effects of adding different levels of linseed oil（LSO） in Leymus chinensis meal on gas production,rumen fermentation, and fatty acids in fermentation fluid were investigated using in vitro procedure. Treatments were control, Leymus chinensls meal containing 5% or 10% of I_SO and cultured 48 h in vitro. The total gas production,methane and hydrogen production were measured at 12,24 and 48 h. After 48 h, rumen fermentation parameters and fatty acids in fer- mented liquid were measured. The results showed that LSO significantly reduced the gas and methane production, im- proved rumen fermentation and increased the hydrogen preduction;LSO significantly increased the total volatile fatty acid （TVFA） ,the proportion of propionate and butyrate, and significantly reduced the pH and ammonia nitrogen concentra- tion;At the same time,I_SO increased the proportion of conjugated linoleic acid and other unsaturated fatty acid which were beneficial to human health. In conclusion, addition of LSO in the forage can suppress methane production, improve rumen fermentation and increase the concentration of polyunsaturated fatty acids（PUFA）.

关 键 词：甲烷 瘤胃发酵 脂肪酸 体外 

分 类 号：R827[医药卫生—临床医学]