番茄斑萎病毒YN-1株系G_N/G_C基因的克隆与分析  

Cloning and sequence analysis of the G_N/G_C gene of Tomato spotted wilt virus YN-1

作　　者：孙书娥[1] 李萌[2,3] 朱春晖[2,3] 谭新球[2,3] 刘勇[1,2,3] 张德咏[1,2,3] 

机构地区：[1]湖南农业大学植保学院,长沙410128 [2]湖南省农业科学院植物保护研究所,长沙410125 [3]园艺作物病虫害治理湖南省重点实验室,长沙410125

出　　处：《植物保护》2014年第1期65-69,共5页Plant Protection

基　　金：国家自然科学基金(31171816;30871619);国家公益性行业(农业)科研专项(201303028);湖南省应用基础研究计划重点项目(2012FJ2009)

摘　　要：作者以TSWV YN-1株系为研究材料,克隆了其GN/GC基因,测序后与GenBank中TSWV其他株系GN/GC基因进行比对,结果显示:YN-1GN/GC基因全长3 408bp,编码1 135个氨基酸,构建的系统发育树可分为4个群,YN-1属于群Ⅱ,与源自西班牙的SPAIN-2株系的GN/GC基因核苷酸相似性最高,达98.88%,氨基酸相似性达98.85%。YN-1株系N基因和GN/GC基因的起源不同,我们推断YN-1可能是一个发生重排(reassortment)的新株系,是不同地理起源的株系在共同传播过程中发生了重排的结果,具体原因有待进一步研究。In this study, we cloned the complete nucleotide sequence of GN/GC gene of TSWV YN-1 strain, and analyzed its phylogenetic relationship to other TSWV isolates from GenBank. The results showed that the com- plete nucleotide sequence of GN/Gc gene was 3 408 nt and encoded 1135 aa, and the phylogenetic tree of the com- plete nucleotide sequence of the GN/Gc gene of TSWV isolated from different hosts in different geographic origins in the world, divided into four groups, were constructed, where TSWV YN-1 was in group Ⅱ, and the complete nucleotide and amino acid sequences of GN/Gc exhibited 98.88% and 98.85% identity with SPAIN-2 （accession number： AY744493） isolated from tomato in Spain, respectively. Because the origins of N gene and GN/GC gene of TSWV YN-1 were different, we inferred that TSWV YN-1 might be a new strain, resulted from a reassortment event happened in co-transmission between different strains of geographic origins. The mechanism needs to be studied further,

关 键 词：番茄斑萎病毒 GN GC基因 克隆 分析 

分 类 号：S432.41[农业科学—植物病理学]